Background The incidence of invasive mycoses is increasing worldwide. PCR-RFLP was applied to the identification of 10 reference strains and 90 cultures of agents of invasive mycoses. In addition, the new approach was applied to detect fungal agents in 120 biological samples (blood, cerebrospinal fluid a...
Background CDIPT (CDP-diacylglycerol-inositol 3-phosphatidyltransferase, EC 2.7.8.11) was found on the cytoplasmic side of the Golgi apparatus and the endoplasmic reticulum. It was an integral membrane protein performing the last step in the de novo biosynthesis of phosphatidylinositol (PtdIns). In recen...
Bovinos/genética,
Industria de la Carne,
Polimorfismo Genético,
Expresión Génica,
CDP-Diacilglicerol-Inositol 3-Fosfatidiltransferasa/genética,
CDP-Diacilglicerol-Inositol 3-Fosfatidiltransferasa/metabolismo,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Polimorfismo de Longitud del Fragmento de Restricción,
Carne/análisis,
Análisis de Secuencia de ADN,
Genotipo,
Electroforesis en Gel de Agar
This article describes the first detection of Cytauxzoon felis, using molecular techniques, in a naturally infected domestic cat from Brazil, South America. Coinfection with 'Candidatus Mycoplasma haemominutum' was also found. The molecular identification of the piroplasmid species was performed by Polym...
Introducción: Entre los factores de riesgo para cáncer laríngeo (CL) son relevantes el consumo de tabaco y alcohol. Estos xenobióticos son metabolizados por un grupo de enzimas, entre las cuales están CYP1A1 y GSTM1, cuyas variantes polimórficas se postulan como factores de riesgo para esta enferme...
Fumar Tabaco/efectos adversos,
Citocromo P-450 CYP1A1/genética,
Neoplasias Laríngeas/genética,
Polimorfismo Genético,
Estudios de Casos y Controles,
Carcinoma de Células Escamosas/genética,
Factores de Riesgo,
Chile/epidemiología,
Glutatión Transferasa/genética,
Fumar Tabaco/genética,
Proyectos Piloto,
Biomarcadores,
Neoplasias Laríngeas/enzimología,
Carcinoma de Células Escamosas/enzimología,
Polimorfismo de Longitud del Fragmento de Restricción
Background: We investigated the polymorphisms of the bovine chemokine receptor-like 1(CMKLR1) gene. The coding region of CMKLR1 was screened in Qinchuan cattle by PCR-RFLP technology. Results: In this study, we discovered two single nucleotide polymorphisms (SNPs) (264G > C and 762C > T) in the coding re...
The 34C>G (Prol2Ala) polymorphic variant of the peroxisome proliferator-activated receptor-γ2 (PPAR-γ2) gene has been associated with polycystic ovary syndrome (PCOS). However, the results between populations are contradictory. Thus, in the present study was investigated the possible associatio...
Síndrome del Ovario Poliquístico/genética,
PPAR gamma/genética,
Chile,
Reacción en Cadena de la Polimerasa/métodos,
Polimorfismo de Longitud del Fragmento de Restricción,
ADN/análisis,
Genotipo,
Lípidos/sangre,
Glucemia/análisis,
Ácido Úrico/sangre,
Colorimetría
The increasing expansion of agro-industrial activity has led to the accumulation of a large quantity of lignocellulosic residues all over the world. In particular, large quantities of rice straw (300.000 t) and citric bagasse (50.000 t) are annually produced in Uruguay. In this work we present the study ...
Pleurotus/metabolismo,
Alimentación Animal,
Pleurotus/química,
Residuos Industriales,
Agricultura,
Polimorfismo de Longitud del Fragmento de Restricción,
Reacción en Cadena de la Polimerasa,
Uruguay,
Valor Nutritivo,
Biodegradación Ambiental,
Coliformes,
Rumiantes,
Escherichia coli/aislamiento & purificación,
Oryza
Little is known about transmission and drug resistance of tuberculosis (TB) in Bauru, State of São Paulo. The objective of this study was to evaluate risk factors for transmission of Mycobacterium tuberculosis strains in this area. Strains were collected from patients attended at ambulatory services in ...
Mycobacterium tuberculosis,
Infecciones Oportunistas Relacionadas con el SIDA,
Tuberculosis Pulmonar,
Antituberculosos,
Rifampin,
Isoniazida,
Mycobacterium tuberculosis,
Mycobacterium tuberculosis,
Polimorfismo de Longitud del Fragmento de Restricción,
Reacción en Cadena de la Polimerasa,
Dermatoglifia del ADN,
Genotipo,
Infección Hospitalaria,
Pruebas de Sensibilidad Microbiana,
Factores de Riesgo,
Brasil
CONTEXT: Human DNA identification is a powerful tool for paternity cases as well as for criminal investigation, in which biological evidence is typed after collection from crime scenes and for the identification of human remains. OBJECTIVE: Identification of a criminal in a rape case with 4 suspects usin...