La espectrometría de masas (EM) (matrix assisted laser desorption ionization-time of flight) MALDI-TOF demostró ser una herramienta robusta para la identificación de numerosos grupos taxonómicos. No obstante, presenta limitaciones. Una ventaja clave de la técnica es la flexibilidad para la incorpora...
Técnicas Bacteriológicas,
Burkholderia/aislamiento & purificación,
Bases de Datos Factuales,
Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónMétodos,
Algoritmos,
Proteínas Bacterianas/análisis,
Burkholderia/química,
Burkholderia/clasificación,
Infecciones por Burkholderia/complicaciones,
Infecciones por Burkholderia/microbiología,
Fibrosis Quística/complicaciones,
Fibrosis Quística/microbiología,
Reproducibilidad de los Resultados,
Especificidad de la Especie
Background: The bioethanol produced from biomass is a promising alternative fuel. The lignocellulose from marginal areas or wasteland could be a promising raw material for bioethanol production because it is present in large quantities, is cheap, renewable and has favorable environmental properties. Desp...
Background: Yarrowia lipolytica is a nonconventional, dimorphic yeast with multiple biotechnological applications. Considering the size of Y. lipolytica cells and a plethora of its morphological forms (spherical cells or hyphae and pseudohyphae), it is highly difficult to select a suitable carrier for th...
Yarrowia/metabolismo,
Yarrowia/química,
Inmovilización,
Celulosa/metabolismo,
Aceites de Plantas,
Levaduras,
Emulsiones,
Nanoestructuras,
Polímeros,
Biotecnología,
Porosidad
Background: Sugars from sweet sorghum stalks can be used to produce ethanol and also to grow oleaginous yeasts. Instead of two separate processes, in this paper we propose a different route producing ethanol and microbial oil in two consecutive fermentation steps. Results: Three yeasts were compared in t...
Etanol/metabolismo,
Biocombustibles/microbiología,
Sorghum/química,
Fermentación,
Jugos de Frutas y Vegetales,
Zumos,
Levaduras,
Trichosporon,
Lipomyces,
Saccharomyces cerevisiae,
Azúcares
Background: The aim of this work was to purify and characterize exo-β-1,3-glucanase, namely, TtBgnA, from the thermophilic fungus Thielavia terrestris Co3Bag1 and to identify the purified enzyme. Results: The thermophilic biomass-degrading fungus T. terrestris Co3Bag1 displayed β-1,3-glucanase activity...
Sordariales/enzimología,
Glucano 1,3-beta-Glucosidasa/química,
Glucano 1,3-beta-Glucosidasa/aislamiento & purificación,
Espectrometría de Masas en Tándem,
Celulasas,
Electroforesis en Gel de Poliacrilamida,
Pruebas de Enzimas,
Concentración de Iones de Hidrógeno,
Estabilidad de Enzimas,
Temperatura
González-Torres, Maykel;
Guzmán-Beltrán, Silvia;
Mata-Gómez, Marco A;
González-Valdez, José;
Leyva-Gómez, Gerardo;
Melgarejo-Ramírez, Yaaziel;
Brostow, Witold;
Velasquillo, Cristina;
Zúñiga-Ramos, Joaquín;
Rodríguez-Talavera, Rogelio.
Background: The search for innovative anti-tubercular agents has received increasing attention in tuberculosis chemotherapy because Mycobacterium tuberculosis infection has steadily increased over the years. This underlines the necessity for new methods of preparation for polymer-drug adducts to treat th...
Polietilenglicoles/química,
Polietilenglicoles/farmacología,
Isoniazida/química,
Isoniazida/farmacología,
Antituberculosos/química,
Antituberculosos/farmacología,
Mycobacterium tuberculosis/efectos de los fármacos,
Técnicas In Vitro,
Polímeros,
Rayos gamma,
Espectrometría Raman,
Profármacos,
Polimerizacion
Background: Pretreatment is the critically important step for the production of ethanol from lignocelluloses. In this study, hardwood birch (Betula pendula) and softwood spruce (Norway spruce) woods were pretreated with a newly synthesized morpholinium ionic liquid, 1-H-3-methylmorpholinium chloride ([HM...
Betula/metabolismo,
Cloruros/química,
Etanol/metabolismo,
Lignina/metabolismo,
Celulosa/metabolismo,
Abies,
Biocombustibles,
Hidrólisis,
Fermentación,
25580,
Etanol/síntesis química
One of the key functions of the hepatobiliary system is bile formation. Aquaporins (AQPs) are likely to play a role in water transport that is essential for appropriate hepatobiliary tract function. The increasing prevalence of fatty liver parallels the rise of obesity and its complications over the past...
A small amount of acetic acid (AA), a common preservative, has been shown to increase contamination in cadaveric tissue, while larger concentrations can lead to the tissue becoming hard, especially in fresh brains. This study attempted to optimize the concentration of AA to be used in the cranial cavity ...
Talebi, Ali;
Sadighi-Gilani, Mohammad Ali;
Koruji, Morteza;
Ai, Jafar;
Navid, Shadan;
Rezaie, Mohammad Jafar;
Jabari, Ayob;
Ashouri-Movassagh, Sepideh;
Khadivi, Farnaz;
Salehi, Majid;
Hoshino, Yumi;
Abbasi, Mehdi.
Spermatogonial stem cells (SSCs) have self-renewal and differentiation capacity essential for sperm production throughout the male reproductive life. The electrospun polycaprolactone/gelatin (PCL/Gel) nanofibrous scaffold mimics important features of the extracellular matrix (ECM), which can provide a pr...
Proliferación Celular/fisiología,
Diferenciación Celular/fisiología,
Espermatogonias/citología,
Espermatogonias/metabolismo,
Animales Recién Nacidos,
Nanofibras/química,
Poliésteres/química,
Andamios del Tejido,
Técnica del Anticuerpo Fluorescente,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Supervivencia Celular,
Proliferación Celular/genética,
Diferenciación Celular/genética
