ABSTRACT Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental po...
Basidiomycota/clasificación,
Basidiomycota/genética,
Basidiomycota/aislamiento & purificación,
Basidiomycota/metabolismo,
Benzo(a)pireno/química,
Benzo(a)pireno/metabolismo,
Biodegradación Ambiental,
Sistema Enzimático del Citocromo P-450/genética,
Sistema Enzimático del Citocromo P-450/metabolismo,
Proteínas Fúngicas/genética,
Proteínas Fúngicas/metabolismo,
Filogenia,
Hidrocarburos Policíclicos Aromáticos/química,
Hidrocarburos Policíclicos Aromáticos/metabolismo,
Pirenos/química,
Pirenos/metabolismo,
Agua de Mar/microbiología
ABSTRACT We examined microbial communities from enriched fine and retorted shale particles using sequencing of V4 variable region of 16S rRNA. High number of microbial genera was found in both enriched shale by-products that were dominate by Actinobacteria, Firmicutes and Proteobacteria, showing differen...
Bacterias/clasificación,
Bacterias/genética,
Bacterias/aislamiento & purificación,
Biodiversidad,
Brasil,
ADN Bacteriano/genética,
Sedimentos Geológicos/química,
Sedimentos Geológicos/microbiología,
Microbiota,
Filogenia,
ARN Ribosómico 16S/genética,
Residuos/análisis
ABSTRACT GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM)...
ABSTRACT The present study aimed to compare two MALDI-TOF identification methods [(a) direct sample identification after pre-incubation; or (b) use of bacteria isolated on pre-culture)] to standard, traditional bench microbiology. A total of 120 quarter milk samples from 40 Holstein lactating cows were s...
Técnicas de Tipificación Bacteriana/métodos,
Mastitis Bovina/microbiología,
Mastitis Bovina/fisiopatología,
Leche/química,
Leche/microbiología,
Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción/métodos,
Staphylococcus/química,
Staphylococcus/genética,
Staphylococcus/aislamiento & purificación,
Streptococcus/química,
Streptococcus/genética,
Streptococcus/aislamiento & purificación
ABSTRACT The presence of mycotoxins or related fungi in animal feed is a major problem for animal and human health. Silage and concentrated feed samples were collected from 21 dairy farms in the Western part of Paraná state in Southern Brazil. Water activity and pH of all samples were measured, and each...
Aflatoxinas/metabolismo,
Alimentación Animal/análisis,
Alimentación Animal/microbiología,
Aspergillus/clasificación,
Aspergillus/genética,
Aspergillus/aislamiento & purificación,
Aspergillus/metabolismo,
Brasil,
Contaminación de Alimentos/análisis,
Ensilaje/clasificación,
Ensilaje/microbiología
ABSTRACT Carnivorous plant species, such as Utricularia spp., capture and digest prey. This digestion can occur through the secretion of plant digestive enzymes and/or by bacterial digestive enzymes. To comprehend the physiological mechanisms of carnivorous plants, it is essential to understand the micro...
ABSTRACT The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an "eco-friendly agricultural practice". Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea (Vigna unguiculata) inoculation in Brazil and showed remarkable r...
Inoculantes Agrícolas/clasificación,
Inoculantes Agrícolas/genética,
Inoculantes Agrícolas/aislamiento & purificación,
Inoculantes Agrícolas/fisiología,
Bradyrhizobium/clasificación,
Bradyrhizobium/genética,
Bradyrhizobium/aislamiento & purificación,
Bradyrhizobium/fisiología,
Brasil,
ADN Bacteriano/genética,
Evolución Molecular,
Genoma Bacteriano,
Genómica,
Filogenia,
ARN Ribosómico 16S/genética,
Nódulos de las Raíces de las Plantas/microbiología,
Simbiosis,
Vigna/microbiología,
Vigna/fisiología
ABSTRACT Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon deg...
Avicennia/metabolismo,
Avicennia/microbiología,
Bacterias/clasificación,
Bacterias/genética,
Bacterias/aislamiento & purificación,
Bacterias/metabolismo,
Biodegradación Ambiental,
ADN Bacteriano/genética,
Hidrocarburos/metabolismo,
Petróleo/análisis,
Contaminación por Petróleo/análisis,
Plásmidos/genética,
Plásmidos/metabolismo,
ARN Ribosómico 16S/genética,
Rizosfera,
Contaminantes del Suelo/análisis,
Contaminantes del Suelo/metabolismo
ABSTRACT Anaerobic digestion is important for the management of livestock manure with high ammonia level. Although ammonia effects on anaerobic digestion have been comprehensively studied, the molecular mechanism underlying ammonia inhibition still remains elusive. In this study, based on metatranscripto...
Amoníaco/metabolismo,
Anaerobiosis,
Bacterias/clasificación,
Bacterias/genética,
Bacterias/aislamiento & purificación,
Bacterias/metabolismo,
Reactores Biológicos/microbiología,
Ácidos Grasos Volátiles/metabolismo,
Metano/metabolismo,
Microbiota,
Transcripción Genética
ABSTRACT In order for the use of biological carotenoids to become feasible, it is necessary to have adequate low cost sources and improved methods of cultivation. The aim of this study was to evaluate the effect of supplementation with nitrogen, phosphorus, zinc, and magnesium, on the biomass and caroten...
Biomasa,
Carotenoides/biosíntesis,
Medios de Cultivo/síntesis química,
Medios de Cultivo/química,
Medios de Cultivo/metabolismo,
Nitrógeno/metabolismo,
Rhodotorula/genética,
Rhodotorula/crecimiento & desarrollo,
Rhodotorula/metabolismo,
Saccharum/metabolismo,
Saccharum/microbiología
