LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Cloning, characterization and expression of Peking duck fatty acid synthase during adipocyte differentiation

Ding, Fang; Yuan, Xin; Li, Qingqing; Sun, Wenqiang; Gan, Chao; He, Hua; Song, Chenling; Wang, Jiwen.

Electron. j. biotechnol; 17 (6), 2014

Background Fatty acid synthase (FAS) is a key enzyme of de novo lipogenesis (DNL), which has been cloned from several species: Gallus gallus, Mus musculus, Homo sapiens, but not from Anas platyrhynchos. The current study was conducted to obtain the full-length coding sequence of Peking duck FAS and inves...

Ácido Graso Sintasas/genética, Ácido Graso Sintasas/metabolismo, Lipogénesis, Tejido Adiposo/metabolismo, Diferenciación Celular, Ácido Oléico, Patos, Análisis de Secuencia, ARN/análisis, ADN Complementario/síntesis química, Supervivencia Celular, Expresión Génica, Biología Computacional, Filogenia, Clonación Molecular

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Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress

Miao, Hongxia; Wang, Yuan; Liu, Juhua; Jia, Caihong; Hu, Wei; Sun, Peiguang; Jin, Zhiqiang; Xu, Biyu.

Electron. j. biotechnol; 17 (6), 2014

Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis...

Musa/genética, Clonación Molecular, Proteínas de Plantas/genética, Ácido Abscísico, Reguladores del Crecimiento de las Plantas, ADN Complementario/síntesis química, Estrés Salino, Proteínas de Plantas/metabolismo, Arabidopsis, Reacción en Cadena en Tiempo Real de la Polimerasa, Análisis de Secuencia, ARN/análisis, Plantas Modificadas Genéticamente, Tolerancia a la Sal

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A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization

Wei, Lei; Yin, Li; Hu, Xiaole; Xu, Ying; Chen, Fang.

Electron. j. biotechnol; 17 (6), 2014

Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pat...

Jatropha/enzimología, Isomerasas de Doble Vínculo Carbono-Carbono/metabolismo, Isomerasas de Doble Vínculo Carbono-Carbono/genética, Hemiterpenos/metabolismo, Hemiterpenos/genética, Southern Blotting, Clonación Molecular, Jatropha/química, ADN Complementario/síntesis química, ARN/aislamiento & purificación, Filogenia, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa, Expresión Génica, Cloroplastos

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