Abstract The seroprevalence of Sarcocystis spp. and Toxoplasma gondii was researched in swine raised in Santa Maria, RS, Brazil. Serum samples from 84 pigs from 31 farms were tested using indirect immunofluorescence assay (IFA) for both agents. Additionally, 53 samples of pork sausages and tissues destin...
Porcinos/parasitología,
Sarcocistosis/epidemiología,
Sarcocistosis/diagnóstico,
Toxoplasmosis Animal/epidemiología,
Toxoplasmosis Animal/diagnóstico,
Sarcocistosis/veterinaria,
Enfermedades de los Porcinos/epidemiología,
Enfermedades de los Porcinos/diagnóstico,
Sarcocystis/genética,
Toxoplasma/genética,
Enfermedades de los Porcinos/parasitología,
Anticuerpos Antiprotozoarios/análisis,
Toxoplasma/inmunología,
Sarcocystis/inmunología,
Prevalencia,
Estudios Seroepidemiológicos,
Carne de Cerdo/parasitología,
ADN Protozoario/inmunología
Abstract INTRODUCTION: The objective of this study was to evaluate the performance of filter paper (FP) for lesion scraping collection in a polymerase chain reaction (PCR) for cutaneous leishmaniasis (CL) diagnosis. METHODS: Lesion scrapings from 48 patients were collected and analyzed for PCR. RESULT...
Abstract This study aimed to identify members of the Sarcocystidae family in naturally infected wild birds at a rescue center in the state of Minas Gerais, southeastern Brazil. The heart and brain of 44 wild birds were evaluated by bioassay in mice to detect T. gondii, and extracted DNA was used for nest...
Bioensayo,
Enfermedades de las Aves/epidemiología,
Enfermedades de las Aves/parasitología,
Aves,
Brasil,
Coccidiosis/epidemiología,
ADN Protozoario,
Reacción en Cadena de la Polimerasa,
ARN Ribosómico 18S/genética,
Sarcocystidae/genética,
Sarcocystis/genética,
Toxoplasma/genética,
Toxoplasmosis Animal/epidemiología
Abstract Leishmaniasis is a zoonotic disease caused by over 20 species of protozoan parasites of the genus Leishmania. Infection is commonly spread by sandflies and produces a wide spectrum of clinical signs and symptoms. Therefore, from an epidemiological and therapeutic standpoint, it is important to d...
Abstract Molecular methods such as Copro-PCR stand out in the diagnosis of T. gondii, because they are highly sensitive and specific, and can distinguish T. gondii from other morphologically similar coccids. The purpose was the detection of Toxoplasma gondii copro-prevalence by polymerase chain reaction ...
Silva, Victoria Luiza de Barros;
Almeida, Sayanne Luns Hatum de;
Maia, Maerle Oliveira;
Santos, Tarcísio Ávila;
Pavelegini, Lucas Avelino Dandolini;
Zaffalon, Gabriela Brocco;
Marcili, Arlei;
Morgado, Thaís Oliveira;
Dutra, Valéria;
Nakazato, Luciano;
Pacheco, Richard de Campos.
Abstract To a better insight into the epidemiology and genetic diversity of protozoan hemoparasites infections in wild mammals, this study aimed to the post mortem detection of DNA from species of the order Piroplasmida (Babesia sp., Cytauxzoon sp., and Theileria sp.) and suborder Adelorina (Hepatozoon s...
Abstract Toxoplasmosis occurs worldwide causing economic losses to the animal production and problems to the public health. The study aimed to detect Toxoplasma gondii and Sarcocystis spp.in 141 meat products from commercial meat cuts of pork, beef, and kibbeh sold in commercial markets from Botucatu, SP...
Abstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the ...
BACKGROUND The genetic heterogeneity of Leishmania parasites is a major factor responsible for the wide variety of Leishmania-associated manifestations. Consequently, understanding the genetic make-up of Leishmania species using suitable molecular markers is an important component of realising local and...
The present study aimed to evaluate the occurrence of Blastocystis sp. in Brazilian studies over a period of years (2000-2020), as well as point out relevant aspects of this enigmatic organism. We performed a literature search using six sources of international databases. The data were divided into diagn...