LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Nontuberculous mycobacteria in patients with suspected tuberculosis and the genetic diversity of Mycobacterium avium in the extreme south of Brazil

Busatto, Caroline; Vianna, Júlia Silveira; Silva, Ana Barbara Scholante; Basso, Rossana; Silveira, Jussara; Groll, Andrea Von; Ramis, Ivy Bastos; Silva, Pedro Eduardo Almeida da.

J. bras. pneumol; 46 (2), 2020

ABSTRACT Objective: Nontuberculous mycobacteria (NTM) are a heterogeneous group of bacteria that are widely distributed in nature and associated with opportunistic infections in humans. The aims of this study were to identify NTM in patients with suspected tuberculosis who presented positive cultures an...

Proteínas Bacterianas/genética, Técnicas de Tipificación Bacteriana, Brasil, Chaperonina 60/genética, ARN Polimerasas Dirigidas por ADN/genética, Variación Genética, Infecciones por Mycobacterium no Tuberculosas/diagnóstico, Infecciones por Mycobacterium no Tuberculosas/microbiología, Mycobacterium avium/genética, Mycobacterium avium/aislamiento & purificación, Micobacterias no Tuberculosas/aislamiento & purificación

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Detection of multidrug-resistant Mycobacterium tuberculosis strains isolated in Brazil using a multimarker genetic assay for katG and rpoB genes

Café Oliveira, Luita Nice; Muniz-Sobrinho, Jairo da Silva; Viana-Magno, Luiz Alexandre; Oliveira Melo, Sônia Cristina; Macho, Antonio; Rios-Santos, Fabrício.

Braz. j. infect. dis; 20 (2), 2016

Abstract Multidrug-resistant tuberculosis (MDRTB) is a serious world health problem that limits public actions to control tuberculosis, because the most used anti-tuberculosis first-line drugs fail to stop mycobacterium spread. Consequently, a quick detection through molecular diagnosis is essential to r...

ADN Bacteriano, Catalasa/genética, Proteínas Bacterianas/genética, Antituberculosos/farmacología, ARN Polimerasas Dirigidas por ADN/genética, Farmacorresistencia Bacteriana Múltiple/genética, Marcadores Genéticos, Genotipo, Isoniazida/farmacología, Pruebas de Sensibilidad Microbiana, Mutación/genética, Mycobacterium tuberculosis/efectos de los fármacos, Mycobacterium tuberculosis/genética, Reacción en Cadena de la Polimerasa, Valor Predictivo de las Pruebas, Rifampin/farmacología, Sensibilidad y Especificidad, Tuberculosis Resistente a Múltiples Medicamentos/microbiología

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Improved assembly procedure of viral RNA genomes amplified with Phi29 polymerase from new generation sequencing data

Berthet, Nicolas; Descorps-Declère, Stéphane; Nkili-Meyong, Andriniaina Andy; Nakouné, Emmanuel; Gessain, Antoine; Manuguerra, Jean-Claude; Kazanji, Mirdad.

Biol. Res; 49 (), 2016

BACKGROUND: New sequencing technologies have opened the way to the discovery and the characterization of pathogenic viruses in clinical samples. However, the use of these new methods can require an amplification of viral RNA prior to the sequencing. Among all the available methods, the procedure based on...

ARN Polimerasas Dirigidas por ADN/genética, Técnicas de Amplificación de Ácido Nucleico/métodos, Análisis de Secuencia de ARN/métodos, Genoma Viral, ARN Viral, Ensamble de Virus, Alphavirus/genética, Mengovirus/genética, República Centroafricana, Biología Computacional, Mapeo Contig, Valores de Referencia, Reproducibilidad de los Resultados, Programas Informáticos

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