Introducción: La inmunoelectroforesis es una técnica de precipitación que permite la caracterización de muestras biológicas complejas. En el Departamento de Inmunología del Instituto de Ciencias Básicas y Preclínicas Victoria de Girón se cuenta con un antisuero hiperinmune obtenido por inmuniza...
Abstract INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus red...
Abstract INTRODUCTION West Nile virus (WNV) immunoglobulin M (IgM) antibodies have been shown to persist for up to 500 days in certain patients. To evaluate the usefulness of immunoglobulin G (IgG) avidity assessment in the diagnosis of WNV infection, we analyzed 54 WNV IgM- and/or IgG-positive serum s...
Abstract An evaluation was made of the kinetics and avidity of anti-Toxocara antibodies (IgG) in rabbits experimentally infected with embryonated Toxocara canis eggs. Seventeen four month old New Zealand White rabbits were distributed into two groups. In the experimental group, twelve rabbits were infect...
Objetivo: Estuda comparativamente técnicas laboratoriais aplicadas ao diagnóstico da toxoplasmose em suínos abatidos em matadouros de Carapicuíba-Brasil e Lima-Peru, e para padronizar a reaçäo de avidez dos anticorpos IgG anti-Toxoplasma gondii. Material e métodos: Examina 30 amostras de cérebros...
Padroniza e avalia o teste de avidez de anticorpos IgG anti-sarampo, aplicando-se a técnica ELISA e a uréia 7M durante 10 minutos como agente dissociante da ligaçäo antígeno-anticorpo, determinou-se o cut-off do baixo índice de avidez (BIA) para definiçäo de primo-vacinaçäo como 29 por cento e ...
Graft rejection is the major cause of failure of HLA mismatched bone marrow transplantation because of residual host immunity. we have proposed to use a monoclonal murine antibody specific for the LFA-1 molecule (25-3) to prevent graft failure in HLA mismatched bone marrow transplantation (BMT). The rati...
