Drought is one of the major constraints in durum wheat production in the Mediterranean Basin. In order to overcome this problem, the genetic transformation of durum wheat is one of the choices for improvement. However, the recalcitrance to Agrobacterium-mediated transformation in durum wheat (Triticum tu...
Bactérias regulam a expressão de diversos fenótipos de acordo com a sua densidade populacional, em um comportamento conhecido como quorum sensing. Em micro-organismos de origem alimentar, o quorum sensing pode influenciar na formação de biofilmes, produção de toxinas e de enzimas hidrolíticas. Em...
Background: Hydrophobins are small proteins secreted by filamentous fungi, which show a highly surface activity. Because of the signally self-assembling abilities and surface activities, hydrophobins were considered as candidates in many aspects, for example, stabilizing foams and emulsions in food produ...
Proteínas Fúngicas/genética,
Proteínas Fúngicas/metabolismo,
Clonación Molecular,
Lentinula/genética,
Lentinula/metabolismo,
Basidiomycota/metabolismo,
Emulsionantes,
Levaduras,
Agrobacterium tumefaciens/metabolismo,
Southern Blotting,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Gliceraldehído-3-Fosfato Deshidrogenasas/metabolismo,
Electroforesis en Gel de Poliacrilamida,
Basidiomycota/enzimología,
Transformación Genética,
Microscopía Fluorescente,
Análisis de Secuencia
Liu, Juhua;
Gao, Pengzhao;
Sun, Xiuxiu;
Zhang, Jing;
Sun, Peiguang;
Wang, Jiashui;
Jia, Caihong;
Zhang, Jianbin;
Hu, Wei;
Xu, Biyu;
Jin, Zhiqiang.
Background: Banana (Musa spp.) is an important staple food, economic crop, and nutritional fruit worldwide. Conventional breeding has been seriously hampered by their long generation time, polyploidy, and sterility of most cultivated varieties. Establishment of an efficient regeneration and transformatio...
Musa/crecimiento & desarrollo,
Musa/genética,
Transformación Genética,
Plantas Modificadas Genéticamente,
Regeneración,
Agrobacterium tumefaciens/fisiología,
Musa/microbiología,
Organogénesis de las Plantas,
Reacción en Cadena de la Polimerasa,
Southern Blotting,
Inmunohistoquímica,
Glucuronidasa
Background: Newcastle disease is an important avian infectious disease that brings about vast economic damage for poultry industry. Transgenic plants represent a cost-effective system for the production of therapeutic proteins and are widely used for the production of poultry vaccines. In an attempt to d...
BACKGROUND: Cancer, being the foremost challenge of the modern era and the focus of world-class investigators, gargantuan research is in progress worldwide to explore novel therapeutic for its management. The exploitation of natural sources has been proven to be an excellent approach to treat or minify t...
Agrobacterium tumefaciens/efectos de los fármacos,
Inhibidores de la Angiogénesis/farmacología,
Antineoplásicos/farmacología,
Extractos Vegetales/farmacología,
Rumex/química,
Saponinas/farmacología,
Saponinas/aislamiento & purificación,
Solventes/química,
Análisis de Varianza,
Pruebas de Sensibilidad Microbiana,
Tumores de Planta,
Reproducibilidad de los Resultados,
Factores de Tiempo
Despite of numerous publications in sunflower genetic transformation, there is no efficient or reproducible protocol with low number of escapes. The latter would indicate that the selection method is not effective. In this work we used Km as selective agent, Agrobacterium tumefaciens EHA105 strain and a ...
Helianthus/genética,
Transformación Genética,
Agrobacterium tumefaciens,
Técnicas In Vitro,
Raíces de Plantas/genética,
Reacción en Cadena de la Polimerasa,
Coloración y Etiquetado,
Kanamicina/administración & dosificación,
Antibacterianos/administración & dosificación,
Transgenes,
Color,
Glucuronidasa,
Regeneración
The aim of this work was to explore the possibility of obtaining transgenic plants of onion varieties cultivated in Argentina, starting from calli induced from mature zygotic embryos, using two strains of Agrobacterium tumefaciens as transfection vectors. Mature embryos from three varieties of 'Valencian...
Cebollas/genética,
Cebollas/enzimología,
Transformación Genética,
Plantas Modificadas Genéticamente,
Regeneración,
Transfección,
Argentina,
Glucuronidasa/metabolismo,
Reacción en Cadena de la Polimerasa,
Agrobacterium tumefaciens,
Cebollas/microbiología
