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Results: 37

Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil

Bertani, Amanda Maria de Jesus; Vieira, Thais; Santos, Carla Adriana dos; Almeida, Elisabete Aparecida de; Camargo, Carlos Henrique; Casas, Monique Ribeiro Tiba.
Sci Rep; 13 (1), 2023
This study analyzes the genomic findings of the first report of Salmonella isolate carrying the blaCTX-M-55 gene, recovered from a bacteremic patient from Brazil. A bacterial isolate positive for the blaCTX-M-55 gene was submitted to antimicrobial susceptibility testing by disk diffusion and epsilometric...
Análisis de Secuencia, Ampicilina, Ceftazidima, Secuenciación Completa del Genoma
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Mutación heterocigota, autosómica recesiva del gen RARS2 en una paciente colombiana de padres no consanguíneos

Bendeck, Joanna L; Villamizar, Ives; Prieto, Carolina; Celis, Luis G.
Arch. argent. pediatr; 120 (1), 2022
Las nuevas metodologías de secuenciación masiva han permitido caracterizar e identificar variantes genéticas asociadas a diferentes patologías. En este trabajo se presenta el caso de una paciente con una mutación del gen RARS2 que codifica la enzima arginino-ARNt ligasa para la codificación de prot...
Arginino-ARNt Ligasa/genética, Colombia, Imagen por Resonancia Magnética, Mutación, Calidad de Vida, Análisis de Secuencia
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Studies on the enzymatic specificity of diguanylate cyclases domains and the production of new second messengers

Justo Arevalo, Santiago.
In the first chapter, studies on substrate recognition and enzymatic activity of GGDEF domains are presented. Many proteins containing GGDEF domains are diguanylate cyclases (DGCs, EC 2.7.7.65), enzymes that catalyze the conversion of 2 GTP molecules into the second messenger c-di-GMP in prokaryotes. Thi...
Análisis de Secuencia, Bacillus pumilus/clasificación, Caulobacter crescentus/química, Cianuros/efectos adversos, Cinética, Clonación de Organismos/instrumentación, Concentración de Iones de Hidrógeno, Especificidad por Sustrato, Estilo de Vida, Genoma Bacteriano, Aislamiento de Pacientes, Mutación Puntual
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Microbiological profile of raw refrigerated and processed bovine milk at dairy industries from Vale do Taquari, Rio Grande do Sul, Brazil

Müller, Thaís; Lunardi, Luca Madalosso; Maciel, Mônica Jachetti; Rempel, Claudete.
Arq. Inst. Biol. (Online); 89 (), 2022
Milk is an essential food, widely consumed by the population. Brazil is one of the world’s largest producers of milk. Milk quality is influenced by several factors in all its stages of production. The aim of this study was to determine the microbiological profile of refrigerated and processed raw bovin...
Leche/microbiología, Alimentos Crudos/análisis, Análisis de Secuencia/métodos, Alimentos Enfriados, Microbiota, Brasil
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Correlation between the number of false positive variants and the quality of results using Ion Torrent PGMâ„¢ sequencing to screen BRCA genes

Gouvêa Moreira, Tiago César; da Silva Spínola, Pricila; Campos Rezende, Micheline; Moreira de Freitas, Carla Simone; Borges Mury, Fábio; Rodrigues Bonvicino, Cibele; de Andrade Agostinho, Luciana.
Biomédica (Bogotá); 41 (4), 2021
Abstract | Introduction: Next Generation Sequencing (NGS) is cost-effective and a faster method to study genes, but its protocol is challenging. Objective: To analyze different adjustments to the protocol for screening the BRCA genes using Ion Torrent PGM sequencing and correlate the results with the num...
Análisis de Secuencia, ADN, Secuenciación de Nucleótidos de Alto Rendimiento, Genes BRCA1, Genes BRCA2
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Short Tandem Repeats in plants: genomic distribution and function prediction

Zhu, Lin; Wu, Hui; Li, Huayang; Tang, Heng; Zhang, Li; Xu, Houjuan; Jiao, Fangchan; Wang, Nian; Yang, Long.
Electron. j. biotechnol; 50 (), 2021
BACKGROUND: Short Tandem repeats (STRs) existed as popular elements in both eukaryotic and prokaryotic genomes. RESULTS: In this study, we analyzed the characteristics, distributions, and motif features of STRs within whole-genomes of 140 plant species. The results showed that STR density was negatively ...
Plantas/genética, Repeticiones de Microsatélite, Variación Genética, Secuencia de Bases, Análisis de Secuencia
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Identification and sequence analysis of prolactin receptor and its differential expression profile at various developmental stages in striped hamsters

Xue, Huiliang; Xu, Jinhui; Wu, Ming; Chen, Lei; Xu, Laixiang.
Braz. j. med. biol. res; 54 (5), 2021
Prolactin (PRL) plays critical roles in regulation of biological functions with the binding of specific prolactin receptor (PRLR). Revealing the expression patterns of PRLR at different developmental stages is beneficial to better understand the role of PRL and its mechanism of action in striped hamsters...
Cricetinae, ADN Complementario/genética, Hipófisis/metabolismo, Prolactina/genética, Receptores de Prolactina/genética, Receptores de Prolactina/metabolismo, Análisis de Secuencia
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Caracterización de Candida spp. aisladas a partir de urocultivos en la ciudad de Medellín

Duque, Clara M; Sanchez, Diana Marcela; Gaviria, Angela; Vallejo Acosta, Alexandra; Gómez, Beatriz; Gómez, Oscar Mauricio; Rua Giraldo, Alvaro; Hernandez, Orville.
Infectio; 24 (4), 2020
Resumen Candida spp. es un agente etiológico importante en infecciones del tracto urinario, principalmente en población con terapia antimicótica de amplio espectro y con catéteres urinarios. Candida albicans es la especie más frecuente, pero otras especies han surgido como patógenos emergentes. En ...
Laboratorios, Candida albicans, Análisis de Secuencia, Candida, Candida parapsilosis, Catéteres Urinarios, Fluconazol, Infecciones, Infecciones Urinarias, Sistema Urinario, Técnicas de Diagnóstico Urológico
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Dynamic changes of total acid and bacterial communities during the traditional fermentation of Hong Qu glutinous rice wine

Liang, Zhangcheng; Lin, Xiaozi; He, Zhigang; Li, Weixin; Ren, Xiangyun; Lin, Xiaojie.
Electron. j. biotechnol; 43 (), 2020
Background: Hong Qu glutinous rice wine (HQGRW) is brewed under non-aseptic fermentation conditions, so it usually has a relatively high total acid content. The aim of this study was to investigate the dynamics of the bacterial communities and total acid during the fermentation of HQGRW and elucidate the...
Vino/microbiología, Lactobacillus/aislamiento & purificación, Pediococcus/aislamiento & purificación, Acetobacter/aislamiento & purificación, Fermentación, Análisis de Componente Principal, Microbiota, Concentración de Iones de Hidrógeno, Lactobacillus/genética, Lactobacillus/metabolismo, Pediococcus/genética, Pediococcus/metabolismo, Acetobacter/genética, Acetobacter/metabolismo, Análisis de Secuencia, Biología Computacional, Factores de Tiempo, Análisis por Conglomerados, Bacterias/aislamiento & purificación
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Padronização, implantação e caracterização molecular de sapovírus em amostras de pacientes com gastroenterites

Chirelli, Audrey Cilli.
No mundo são registrados 1,7 bilhões de casos de doença diarreica aguda (DDA) por ano, sendo considerada a segunda maior causa de morte em crianças menores de 5 anos de idade. Após a introdução da vacina contra rotavírus (RVA) em diversos países do mundo e o aprimoramento das técnicas de diagnÃ...
Sapovirus, 32511, Análisis de Secuencia
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