LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Leishmania infantum induces high phagocytic capacity and intracellular nitric oxide production by human proinflammatory monocyte

Ribeiro, Christiana Vargas; Rocha, Bruna Fonte Boa; Oliveira, Edward; Teixeira-Carvalho, Andrea; Martins-Filho, Olindo Assis; Murta, Silvane Maria Fonseca; Peruhype-Magalhães, Vanessa.

Mem. Inst. Oswaldo Cruz; 115 (), 2020

BACKGROUND The mechanism of resistance to SbIII in Leishmania is complex, multifactorial and involves not only biochemical mechanisms, but also other elements, such as the immune system of the host. OBJECTIVES In this study, putative changes in the immunological profile of human monocytes infected wit...

Antimonio/farmacología, Antiprotozoarios/farmacología, Resistencia a Medicamentos, Citometría de Flujo, Inmunidad Innata, Leishmania braziliensis/efectos de los fármacos, Leishmania braziliensis/inmunología, Leishmania infantum/efectos de los fármacos, Leishmania infantum/inmunología, Monocitos/inmunología, Monocitos/parasitología, Óxido Nítrico/biosíntesis, Fagocitosis/inmunología

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Antimony resistance in Leishmania (Viannia) braziliensis clinical isolates from atypical lesions associates with increased ARM56/ARM58 transcripts and reduced drug uptake

Rugani, Jeronimo Nunes; Gontijo, Célia Maria Ferreira; Frézard, Frédéric; Soares, Rodrigo Pedro; Monte-Neto, Rubens Lima do.

Mem. Inst. Oswaldo Cruz; 114 (), 2019

BACKGROUND In addition to the limited therapeutic arsenal and the side effects of antileishmanial agents, drug resistance hinders disease control. In Brazil, Leishmania braziliensis causes atypical (AT) tegumentary leishmaniasis lesions, frequently refractory to treatment. OBJECTIVES The main goal of ...

Antimonio/farmacología, Acuagliceroporinas/metabolismo, Resistencia a Medicamentos/efectos de los fármacos, Resistencia a Medicamentos/genética, Leishmania braziliensis/efectos de los fármacos, Leishmania braziliensis/genética, Leishmaniasis Cutánea/parasitología, Reacción en Cadena en Tiempo Real de la Polimerasa

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Increased thiol levels in antimony-resistant Leishmania infantum isolated from treatment-refractory visceral leishmaniasis in Brazil

Magalhães, Lucas S; Bomfim, Lays GS; Mota, Sthefanne G; Cruz, Geydson S; Corrêa, Cristiane B; Tanajura, Diego M; Lipscomb, Michael W; Borges, Valéria M; Jesus, Amélia R de; Almeida, Roque P de; Moura, Tatiana R de.

Mem. Inst. Oswaldo Cruz; 113 (2), 2018

BACKGROUND Treatment-refractory visceral leishmaniasis (VL) has become an important problem in many countries. OBJECTIVES We evaluated the antimony-resistance mechanisms of Leishmania infantum isolated from VL patients refractory or responsive to treatment with pentavalent antimony. METHODS Strains iso...

Butionina Sulfoximina, Antimonio/farmacología, Resistencia a Medicamentos, Inhibidores Enzimáticos, Pruebas de Sensibilidad Parasitaria, Leishmaniasis Visceral/parasitología

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Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects

Moreira, Douglas de Souza; Xavier, Mariana Vieira; Murta, Silvane Maria Fonseca.

Mem. Inst. Oswaldo Cruz; 113 (12), 2018

Ascorbate peroxidase (APX) is a redox enzyme of the trypanothione pathway that converts hydrogen peroxide (H2O2) into water molecules. In the present study, the APX gene was overexpressed in Leishmania braziliensis to investigate its contribution to the trivalent antimony (SbIII)-resistance phenotype. We...

Antimonio/farmacología, Antiprotozoarios/farmacología, Ascorbato Peroxidasas/metabolismo, Western Blotting, Resistencia a Medicamentos, Regulación Enzimológica de la Expresión Génica, Leishmania braziliensis/efectos de los fármacos, Leishmania braziliensis/enzimología, Estrés Oxidativo, Pruebas de Sensibilidad Parasitaria, Fenotipo, Proteínas Protozoarias/metabolismo

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