Lacrimal gland primary acinar cell culture: the role of insulin
Arq. bras. oftalmol; 79 (2), 2016
ABSTRACT Purpose: The goal of the present study was to establish a protocol for primary culture of lacrimal gland acinar cells (LGACs) and to assess the effect of adding insulin to the culture media. Methods: LGACs were isolated and cultured from lacrimal glands of Wistar male rats. The study outcomes ...
Células Acinares/citología, Células Acinares/efectos de los fármacos, Células Acinares/metabolismo, Carbacol/metabolismo, Recuento de Células/métodos, Separación Celular/métodos, Insulina/metabolismo, Insulina/farmacología, Aparato Lagrimal/citología, Aparato Lagrimal/metabolismo, Peroxidasa/metabolismo, Cultivo Primario de Células/normas, Ratas Wistar