Results: 10

Arabidopsis CK2 family gene CKB3 involved in abscisic acid signaling

Braz. j. biol; 81 (2), 2021
CKB3 is a regulatory (beta) subunit of CK2. In this study Arabidopsis thaliana homozygous T-DNA mutant ckb3 was studied to understand the role of CKB3 in abscisic acid (ABA) signaling. The results shown: CKB3 was expressed in all organs and the highest expression in the seeds, followed by the root. Durin...

Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca

Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous ...

Ectopic expression of Chrysanthemum CDM19 in Arabidopsis reveals a novel function in carpel development

BACKGROUND: APETALA3 (AP3) has significant roles in petal and stamen development in accordance with the classical ABC model. RESULTS: The AP3 homolog, CDM19, from Chrysanthemum morifolium cv. Jinba was cloned and sequenced. Sequence and phylogenetic analyses revealed that CDM19 is of DEF/AP3 lineage poss...

Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis

Biol. Res; 53 (), 2020
BACKGROUND: UV-B signaling in plants is mediated by UVR8, which interacts with transcriptional factors to induce root morphogenesis. However, research on the downstream molecules of UVR8 signaling in roots is still scarce. As a wide range of functional cytoskeletons, how actin filaments respond to UV-B-i...

Expression profiles of five FT-like genes and functional analysis of PhFT-1 in a Phalaenopsis hybrid

Background: Phalaenopsis is an important ornamental flowering plant that belongs to the Orchidaceae family and is cultivated worldwide. Phalaenopsis has a long juvenile phase; therefore, it is important to understand the genetic elements regulating the transition from vegetative phase to reproductive pha...

Effect of nickel chloride on Arabidopsis genomic DNA and methylation of 18S rDNA

Electron. j. biotechnol; 18 (1), 2015
Background In recent years, nickel (Ni) has been widely applied in industrial and agricultural production and has become a kind of environmental pollution. In this study, the effect of nickel chloride (NiCl2) with different concentrations on Arabidopsis genomic stability and DNA methylation has been demo...

Molecular cloning and expression analysis of the MaASR1 gene in banana and functional characterization under salt stress

Electron. j. biotechnol; 17 (6), 2014
Background Abscisic acid (ABA)-, stress- and ripening-induced protein (ASR) is plant-specific hydrophilic transcriptional regulators involved in sucrose stress and wounding in banana. However, it is not known whether banana ASR genes confer salt stress tolerance. The contexts of the study was to analysis...

Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana

Electron. j. biotechnol; 16 (6), 2013
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arab...

Teores de benzilglicosinolato, benzilisotiocianato e expressão da mirosinase durante o desenvolvimento e amadurecimento do mamão papaia (Carica papaya L., var. Sunrise Solo)

O sistema glicosinolato-microsinase faz parte do mecanismo de defesa das plantas, quando o tecido é danificado, os glicosinolatos são degradados pela mirosinase e os compostos tóxicos são liberados. No mamão, o principal composto liberado pela enzima é o benzilisotiocianato (BITC), a partir da degr...