LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Studies on the recombinant production and anticancer activity of thermostable L- asparaginase I from Pyrococcus abyssi

Nadeem, M S; Khan, J A; Al-Ghamdi, M A; Khan, M I; Zeyadi, M A.

Braz. j. biol; 82 (), 2022

L-Asparaginase catalysing the breakdown of L-Asparagine to L-Aspartate and ammonia is an enzyme of therapeutic importance in the treatment of cancer, especially the lymphomas and leukaemia. The present study describes the recombinant production, properties and anticancer potential of enzyme from a hypert...

Antineoplásicos/farmacología, Asparaginasa/biosíntesis, Asparaginasa/farmacología, Células CACO-2, Estabilidad de Enzimas, Escherichia coli/genética, Concentración de Iones de Hidrógeno, Simulación del Acoplamiento Molecular, Pyrococcus abyssi/enzimología, Proteínas Recombinantes/biosíntesis, Proteínas Recombinantes/farmacología, Especificidad por Sustrato

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Enhanced production of glutaminase-free L-asparaginase by marine Bacillus velezensis and cytotoxic activity against breast cancer cell lines

Mostafa, Yasser; Alrumman, Suliman; Alamri, Saad; Hashem, Mohamed; Al-izran, Kholood; Alfaif, Mohammad; Elbehairi, Serag Eldin; Taha, Tarek.

Electron. j. biotechnol; 42 (), 2019

Background: The increasing rate of breast cancer globally requires extraordinary efforts to discover new effective sources of chemotherapy with fewer side effects. Glutaminase-free L-asparaginase is a vital chemotherapeutic agent for various tumor malignancies. Microorganisms from extreme sources, such a...

Bacillus/enzimología, Glutaminasa/metabolismo, Asparaginasa/metabolismo, Neoplasias de la Mama/metabolismo, Neoplasias de la Mama/tratamiento farmacológico, Células Inmovilizadas, Fermentación, Células MCF-7, Asparaginasa/biosíntesis, Pruebas de Enzimas, Cinética, Concentración de Iones de Hidrógeno, Temperatura

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Current applications and different approaches for microbial L-asparaginase production

Cachumba, Jorge Javier Muso; Antunes, Felipe Antonio Fernandes; Peres, Guilherme Fernando Dias; Brumano, Larissa Pereira; Santos, Júlio César Dos; Silva, Silvio Silvério Da.

Braz. j. microbiol; 47 (supl.1), 2016

ABSTRACT L-asparaginase (EC 3.5.1.1) is an enzyme that catalysis mainly the asparagine hydrolysis in L-aspartic acid and ammonium. This enzyme is presented in different organisms, such as microorganisms, vegetal, and some animals, including certain rodent's serum, but not unveiled in humans. It can be us...

Antineoplásicos, Asparaginasa/biosíntesis, Industria Farmacéutica, Fermentación, Microbiología Industrial, Asparaginasa, Industria de Alimentos

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