Abstract Amylases are enzymes involved in starch hydrolysis, generating the most diverse products, such as maltose, glucose and dextrins. This work aimed the study of the production of amylolytic enzymes via solid-state fermentation (SSF) using "crueira", an essentially starchy cassava residue, as substr...
Background: The increasing rate of breast cancer globally requires extraordinary efforts to discover new effective sources of chemotherapy with fewer side effects. Glutaminase-free L-asparaginase is a vital chemotherapeutic agent for various tumor malignancies. Microorganisms from extreme sources, such a...
Bacillus/enzimología,
Glutaminasa/metabolismo,
Asparaginasa/metabolismo,
Neoplasias de la Mama/metabolismo,
Neoplasias de la Mama/tratamiento farmacológico,
Células Inmovilizadas,
Fermentación,
Células MCF-7,
Asparaginasa/biosíntesis,
Pruebas de Enzimas,
Cinética,
Concentración de Iones de Hidrógeno,
Temperatura
Background: Recombinant DNA technology enables us to produce proteins with desired properties and insubstantial amount for industrial applications. Endo-1, 4-β-glucanases (Egl) is one of the major enzyme involved in degradation of cellulose, an important component of plant cell wall. The present study w...
Bacillus/enzimología,
Escherichia coli/metabolismo,
Celulasas/biosíntesis,
Expresión Génica,
Hidrólisis,
Reacción en Cadena de la Polimerasa,
Clonación Molecular,
Celulasas/metabolismo,
Celulasas/aislamiento & purificación,
Células Vegetales/enzimología,
Pared Celular/enzimología,
Estabilidad de Enzimas,
Concentración de Iones de Hidrógeno,
Temperatura
Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. Results: To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant...
Isomaltosa/metabolismo,
Oligosacáridos/metabolismo,
Enzimas/metabolismo,
Almidón/metabolismo,
alfa-Amilasas/metabolismo,
beta-Amilasa/metabolismo,
Glucosidasas/metabolismo,
Bacillus/enzimología,
Aspergillus niger/enzimología,
Oligosacáridos/biosíntesis,
Licuefacción,
Concentración de Iones de Hidrógeno,
Glicosilación,
Temperatura,
Glicósido Hidrolasas/metabolismo,
Fermentación
Background: Xylanase from bacteria finds use in prebleaching process and bioconversion of lignocelluloses into feedstocks. The xylanolytic enzyme brings about the hydrolysis of complex biomolecules into simple monomer units. This study aims to optimize the cellulase-free xylanase production and cell biom...
Background: Agro-wastes were used for the production of fibrinolytic enzyme in solid-state fermentation. The process parameters were optimized to enhance the production of fibrinolytic enzyme from Bacillus halodurans IND18 by statistical approach. The fibrinolytic enzyme was purified, and the properties ...
Background: Lignocellulosic biomass is a renewable, abundant, and inexpensive resource for biorefining process to produce biofuel and valuable chemicals. To make the process become feasible, it requires the use of both efficient pretreatment and hydrolysis enzymes to generate fermentable sugars. Ionic li...
Bacillus/enzimología,
Celulasa/aislamiento & purificación,
Microbiología del Suelo,
Lignina,
Biomasa,
Líquidos Iónicos,
Oryza,
Celulasa/biosíntesis,
Bacillus/metabolismo,
Biocombustibles,
Concentración de Iones de Hidrógeno,
Temperatura,
Hidrólisis
Background Alkaline proteases are among the most important classes of industrial hydrolytic enzymes. The industrial demand for alkaline proteases with favorable properties continues to enhance the search for new enzymes. The present study focused on isolation of new alkaline producing alkaliphilic bacter...
Bacillus/enzimología,
Bacillus/aislamiento & purificación,
Proteínas Bacterianas/metabolismo,
Endopeptidasas/metabolismo,
Fermentación,
Álcalis,
Cloruro de Sodio,
Lagos,
Temperatura,
Concentración de Iones de Hidrógeno,
Tolerancia a la Sal
Background This paper reports the production of cellulase by thermophilic Bacillus sp. SMIA-2 using sugarcane bagasse and corn steep liquor as substrates. Some biochemical properties of the enzyme were also assessed for the purposes of exploiting its potential in the detergent industry, as well as other ...
A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60ºC and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30 percent v/v each). The activity (assayed by determining the release o...
