Twin-arginine signal peptide of Bacillus licheniformis GlmU efficiently mediated secretory expression of protein glutaminase
Electron. j. biotechnol; 42 (), 2019
Background: Protein glutaminase specifically deamidates glutamine residue in protein and therefore significantly improves protein solubility and colloidal stability of protein solution. In order to improve its preparation efficiency, we exploited the possibility for its secretory expression mediated by t...
Bacillus licheniformis/enzimología, Bacillus subtilis, Escherichia coli, Glutaminasa/metabolismo, Glutaminasa/genética, Señales de Clasificación de Proteína, Arginina, Prostaglandinas A/química, Bacillus licheniformis/genética, Secuencia de Bases, Plásmidos, Mutagénesis Sitio-Dirigida, Ácido Aspártico