Huang, Lin;
Zheng, Dong;
Zhao, Yatong;
Ma, Jieying;
Li, Yanzhen;
Xu, Zehua;
Shan, Mengying;
Shao, Shulin;
Guo, Qingwen;
Zhang, Jie;
Lu, Fuping;
Liu, Yihan.
BACKGROUND: Lipases are extensively exploited in lots of industrial fields; cold-adapted lipases with alkali-resistance are especially desired in detergent industry. Penicillium cyclopium lipase I (PCL) might be suitable for applications of detergent industry due to its high catalytic efficiency at low t...
Penicillium/enzimología,
Estabilidad de Enzimas,
Lipasa/metabolismo,
Álcalis,
Biocatálisis,
Reacción en Cadena de la Polimerasa/métodos,
Frío,
Penicillium/genética,
Lipasa/genética,
Penicillium/aislamiento & purificación,
Lipasa/aislamiento & purificación,
Mutación,
Industria de Detergentes,
Concentración de Iones de Hidrógeno,
Interacciones Hidrofóbicas e Hidrofílicas
ABSTRACT The multi-enzyme complex (crude extract) of white rot fungi Pleurotus ostreatus, Pleurotus eryngii, Trametes versicolor, Pycnosporus sanguineus and Phanerochaete chrysosporium were characterized, evaluated in the hydrolysis of pretreated pulps of sorghum straw and compared efficiency with commer...
Biocatálisis,
Celulasas/química,
Celulasas/metabolismo,
Proteínas Fúngicas/química,
Proteínas Fúngicas/metabolismo,
Hongos/química,
Hongos/enzimología,
Hidrólisis,
Lignina/química,
Lignina/metabolismo,
Tallos de la Planta/química,
Tallos de la Planta/microbiología,
Sorghum/química,
Sorghum/microbiología
Background: Cellulolytic enzymes of microbial origin have great industrial importance because of their wide application in various industrial sectors. Fungi are considered the most efficient producers of these enzymes. Bioprospecting survey to identify fungal sources of biomass-hydrolyzing enzymes from a...
Penicillium/enzimología,
beta-Glucosidasa/biosíntesis,
Hidrólisis,
Celulasa/biosíntesis,
Lignina/metabolismo,
Trichoderma/enzimología,
Biocatálisis,
Fermentación,
Oligosacáridos,
Ecosistema Amazónico,
Concentración de Iones de Hidrógeno,
Temperatura,
Estabilidad de Enzimas,
beta-Glucosidasa/metabolismo,
Celulasa/metabolismo
ABSTRACT Several enzymatic reactions of heteroatom-containing compounds have been explored as unnatural substrates. Considerable advances related to the search for efficient enzymatic systems able to support a broader substrate scope with high catalytic performance are described in the literature. These ...
Background: Invert sugar is used greatly in food and pharmaceutical industries. This paper describes scaling-up batch conditions for sucrose inversion catalyzed by the recombinant Pichia pastoris BfrA4X whole cells expressing Thermotoga maritima invertase entrapped in calcium alginate beads. For the firs...
Pichia/metabolismo,
Pichia/citología,
Hidrólisis,
Biotecnología/métodos,
Sacarosa/metabolismo,
Sacarosa/química,
Reactores Biológicos,
Biocatálisis,
Enzimas Inmovilizadas,
Cinética,
Alginatos,
Thermotoga maritima/enzimología
The biotechnology sector is continually seeking sustainable and more economical bioprocesses. Fermentation media produced with cheap components or wastes reduce production costs. Moreover, if wastes are used, they contribute to avoid environmental pollution. In this work, microbial growth media based on ...
The permeabilization was used to transform microorganisms in cell biocatalysts with high enzymatic activity. The Saccharomyces fragilis IZ 275 yeast cells were permeabilized with ethanol, as permeabilizing agent. To optimize the permeabilization conditions were used the design of Box-Behnken 15 trials (3...
Background β-Glucosidases catalyze the hydrolysis of cellobiose and cellodextrins, releasing glucose as the main product. This enzyme is used in the food, pharmaceutical, and biofuel industries. The aim of this work is to improve the β-glucosidase production by the fungus Lichtheimia ramosa by ...
Background: New enzymes for biotransformations can be obtained by different approaches including directed mutagenesis and in vitro evolution. These mutants have to be efficiently produced for laboratory research on bioreactions as well as for process development. In the framework of a European ERA-IB pro...
Amoníaco-Liasas/metabolismo,
Amoníaco-Liasas/genética,
Aminas/metabolismo,
Transaminasas/metabolismo,
Transaminasas/genética,
Enzimas Inmovilizadas,
Biocatálisis,
Amoníaco-Liasas/química,
Aminas/química,
Transaminasas/química,
Técnicas de Cultivo Celular por Lotes,
Escherichia coli,
Mutación,
Medios de Cultivo,
Reactores Biológicos
The potential of lipases (E.C.3.1.1.3.) as biocatalysts for the production of fatty acid derivatives for the food and nutraceutical industries, such as flavouring esters, fatty acid esters of antioxidants and structured lipids, is enormous, mainly due to their high regio- and stereo-selectivities, in add...
