BACKGROUND: Chinese hamster ovary cell line has been used routinely as a bioproduction factory of numerous biopharmaceuticals. So far, various engineering strategies have been recruited to improve the production efficiency of this cell line such as apoptosis engineering. Previously, it is reported that t...
BACKGROUND: Chinese hamster ovary (CHO) cells are the workhorse for obtaining recombinant proteins. Proteomic studies of these cells intend to understand cell biology and obtain more productive and robust cell lines for therapeutic protein production in the pharmaceutical industry. Because of the great i...
Proteínas Recombinantes,
Células CHO,
Precipitación Química,
Proteómica/métodos,
Separación Celular,
Solubilidad,
Electroforesis en Gel de Poliacrilamida,
Técnicas de Cultivo de Célula,
Acetona,
Cloroformo,
Metanol,
Ácido Tricloroacético
BACKGROUND: Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell In the commercial-scale production of biopharmaceutical proteins. Modification of genes involved in apoptosis may improve the productivity of CHO cells. Executive caspases, including caspases 3 and 7, play critic...
Cancer is a multifactorial disease that constitutes a serious public health problem worldwide. Prostate cancer advanced stages are associated with the development of androgen-independent tumors and an apoptosis-resistant phenotype that progresses to metastasis. By studying androgen-independent lymphoid n...
Apoptosis/fisiología,
Proteínas Reguladoras de la Apoptosis/aislamiento & purificación,
Proteínas Reguladoras de la Apoptosis/metabolismo,
Células CHO,
Calcio/metabolismo,
Canales de Calcio/metabolismo,
Cricetulus,
ADN Complementario/aislamiento & purificación,
Óvulo/metabolismo,
Neoplasias de la Próstata/metabolismo,
Neoplasias de la Próstata/patología,
ARN Mensajero/metabolismo,
Xenopus laevis
Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines. Multiple copies of integrated genes by retrovector transduction res...
O fator de crescimento transformante beta tipo 1, TGF-β1, é uma proteína extracelular homodimérica secretada por vários tipos celulares, que pode ter ação parácrina ou endócrina. Essa proteína está envolvida em processos celulares de diferenciação, proliferação, mobilidade e formação de ...
O fator de crescimento transformante beta tipo 1, TGF-β1, é uma proteína extracelular homodimérica secretada por vários tipos celulares, que pode ter ação parácrina ou endócrina. Essa proteína está envolvida em processos celulares de diferenciação, proliferação, mobilidade e formação de ...
Background: To reduce costs associated with productivity of recombinant proteins in the biopharmaceutical industry, research has been focused on regulatory principals of growth and survival during the production phases of the cell culture. The main strategies involve the regulation of cell proliferation ...
Células CHO/metabolismo,
Células CHO/fisiología,
Proteínas Recombinantes/metabolismo,
Hipotermia,
Ácido Butírico/metabolismo,
Proliferación Celular,
Activador de Tejido Plasminógeno/metabolismo,
Activador de Tejido Plasminógeno/biosíntesis,
Supervivencia Celular,
Ciclo Celular,
Potencial de la Membrana Mitocondrial
Background Polycosanols derived from plant species have traditionally been used in medicine as antiproliferative agents for treating various viruses (primarily the herpes simplex virus). However, few studies have studied their effects on hyperproliferative cell lines. In this work, the antiproliferative ...
Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure a...
