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Retrovectors packaged in CHO cells to generate GLP-1-Fc stable expression CHO cell lines

Li, Jing; Wei, Suzhen; Cao, Chunlai; Chen, Kangyue; He, Hua; Gao, Guoquan.
Electron. j. biotechnol; 41 (), 2019
Background: Chinese hamster ovary (CHO) cells are the most dependable mammalian cells for the production of recombinant proteins. Replication-incompetent retroviral vector (retrovector) is an efficient tool to generate stable cell lines. Multiple copies of integrated genes by retrovector transduction res...
Células CHO/metabolismo, Proteínas Recombinantes/metabolismo, Vectores de Enfermedades, Retroviridae, Línea Celular, Técnicas de Cultivo Celular por Lotes, Cromatografía en Gel/métodos, Espectrometría de Masas en Tándem, Péptido 1 Similar al Glucagón, Fragmentos Fc de Inmunoglobulinas
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Impact of sodium butyrate and mild hypothermia on metabolic and physiological behaviour of CHO TF 70R cells

Avello, Veronica; Tapia, Bethzabeth; Vergara, Mauricio; Acevedo, Cristian; Berrios, Julio; Reyes, Juan G; Altamirano, Claudia.
Electron. j. biotechnol; 27 (), 2017
Background: To reduce costs associated with productivity of recombinant proteins in the biopharmaceutical industry, research has been focused on regulatory principals of growth and survival during the production phases of the cell culture. The main strategies involve the regulation of cell proliferation ...
Células CHO/metabolismo, Células CHO/fisiología, Proteínas Recombinantes/metabolismo, Hipotermia, Ácido Butírico/metabolismo, Proliferación Celular, Activador de Tejido Plasminógeno/metabolismo, Activador de Tejido Plasminógeno/biosíntesis, Supervivencia Celular, Ciclo Celular, Potencial de la Membrana Mitocondrial
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Advances in improving mammalian cells metabolism for recombinant protein production

Altamirano, Claudia; Berrios, Julio; Vergara, Mauricio; Becerra, Silvana.
Electron. j. biotechnol; 16 (3), 2013
Background: The production of recombinant proteins for therapeutic use represents a great impact on the biotechnology industry. In this context, established mammalian cell lines, especially CHO cells, have become a standard system for the production of such proteins. Their ability to properly configure a...
Proteínas Recombinantes/biosíntesis, Mamíferos/metabolismo, Células/metabolismo, Ingeniería Celular, Células CHO/metabolismo, Glucólisis, Metabolismo Energético, Glutamina/metabolismo
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