BACKGROUND: Nonribosomal peptide synthases (NRPS) can synthesize functionally diverse bioactive peptides by incorporating nonproteinogenic amino acids, offering a rich source of new drug leads. The bacterium Escherichia coli is a well-characterized production host and a promising candidate for the synthe...
BACKGROUND: Pyruvic acid (PA), a vital α-oxocarboxylic acid, plays an important role in energy and carbon metabolism. The oleaginous yeast Yarrowia lipolytica (Y. lipolytica) has considerable potential for the production of PA. An increased NaCl concentration reportedly increases the biomass and PA yiel...
Yarrowia/metabolismo,
Yarrowia/genética,
Ácido Pirúvico/metabolismo,
Mutación,
Fermentación,
Tolerancia a la Sal/genética,
Reactores Biológicos,
Presión Osmótica,
Carbono/metabolismo,
Glicerol/metabolismo,
Levaduras,
Cloruro de Sodio
ABSTRACT To mitigate the deleterious effects of abiotic stress, the use of plant growth-promoting bacteria along with diazotrophic bacteria has been increasing. The objectives of this study were to investigate the key enzymes related to nitrogen and carbon metabolism in the biological nitrogen fixation p...
Actinobacteria/fisiología,
Inoculantes Agrícolas/fisiología,
Aminoácidos/metabolismo,
Bradyrhizobium/fisiología,
Carbono/metabolismo,
Nitrógeno/metabolismo,
Fijación del Nitrógeno,
Raíces de Plantas/crecimiento & desarrollo,
Raíces de Plantas/metabolismo,
Raíces de Plantas/microbiología,
Estrés Salino,
Cloruro de Sodio/análisis,
Cloruro de Sodio/metabolismo,
Microbiología del Suelo,
Vigna/crecimiento & desarrollo,
Vigna/metabolismo,
Vigna/microbiología
ABSTRACT A bacterium isolated from Sterkfontein dam was confirmed to produce bioflocculant with excellent flocculation activity. The 16S rDNA nucleotide sequence analyses revealed the bacteria to have 99% similarity to Streptomyces platensis strain HBUM174787 and the sequence was deposited in the Genbank...
Proteínas Bacterianas/genética,
Proteínas Bacterianas/metabolismo,
Carbono/metabolismo,
Floculación,
Nitrógeno/metabolismo,
Ríos/química,
Streptomyces/química,
Streptomyces/genética,
Streptomyces/aislamiento & purificación,
Streptomyces/metabolismo,
Aguas Residuales/química,
Microbiología del Agua,
Purificación del Agua
Abstract Cellulosimicrobium cellulans CWS2, a novel strain capable of utilizing benzo(a)pyrene (BaP) as the sole carbon and energy source under nitrate-reducing conditions, was isolated from PAH-contaminated soil. Temperature and pH significantly affected BaP biodegradation, and the strain exhibited enha...
Actinobacteria/clasificación,
Actinobacteria/aislamiento & purificación,
Actinobacteria/metabolismo,
Anaerobiosis,
Benzo(a)pireno/metabolismo,
Biotransformación,
Cadmio/metabolismo,
Carbono/metabolismo,
Ácidos Carboxílicos/metabolismo,
Medios de Cultivo/química,
Inhibidores Enzimáticos/metabolismo,
Cromatografía de Gases y Espectrometría de Masas,
Glucosa/metabolismo,
Concentración de Iones de Hidrógeno,
Microbiología del Suelo,
Contaminantes del Suelo/metabolismo,
Temperatura
Background: A new ι-carrageenase-producing strain was screened from mangroves and authenticated as Pseudoalteromonas carrageenovora ASY5 in our laboratory. The potential application of this new strain was evaluated. Results: Medium compositions and culturing conditions in shaking flask fermentation were...
Hidrólisis,
Pseudoalteromonas/enzimología,
Glicósido Hidrolasas/metabolismo,
Proteínas Bacterianas/metabolismo,
Fermentación,
Temperatura,
Concentración de Iones de Hidrógeno,
Carragenina/biosíntesis,
Espectrometría de Masa por Ionización de Electrospray,
Nitrógeno/metabolismo,
Carbono/metabolismo,
Oligosacáridos/biosíntesis
Abstract Although Planktothrix agardhii often produces toxic blooms in eutrophic water bodies around the world, little is known about the fate of the organic matter released by these abundant Cyanobacteria. Thus, this study focused in estimating the bacterial consumption of the DOC and DON (dissolved org...
Abstract Nowadays, it is necessary to search for different high-scale production strategies to produce recombinant proteins of economic interest. Only a few microorganisms are industrially relevant for recombinant protein production: methylotrophic yeasts are known to use methanol efficiently as the sole...
Abstract Sclareol is an important intermediate for ambroxide synthesis industries. Hyphozyma roseonigra ATCC 20624 was the only reported strain capable of degrading sclareol to the main product of sclareol glycol, which is the precursor of ambroxide. To date, knowledge is lacking about the effects of scl...
Ascomicetos/química,
Ascomicetos/genética,
Ascomicetos/metabolismo,
Carbono/metabolismo,
Diterpenos/metabolismo,
Electroforesis en Gel Bidimensional,
Proteínas Fúngicas/química,
Proteínas Fúngicas/metabolismo,
Regulación Fúngica de la Expresión Génica,
Glucosa/metabolismo,
Proteómica
Abstract Antibodies and antibody fragments are nowadays among the most important biotechnological products, and Pichia pastoris is one of the most important vectors to produce them as well as other recombinant proteins. The conditions to effectively cultivate a P. pastoris strain previously genetically m...
Anticuerpos/genética,
Anticuerpos/metabolismo,
Carbono/metabolismo,
Medios de Cultivo/química,
Medios de Cultivo/metabolismo,
Fermentación,
Glicerol/metabolismo,
Microbiología Industrial/métodos,
Lipoproteínas LDL/inmunología,
Pichia/genética,
Pichia/crecimiento & desarrollo,
Pichia/metabolismo,
Proteínas Recombinantes/genética,
Proteínas Recombinantes/metabolismo,
Anticuerpos de Cadena Única/biosíntesis,
Anticuerpos de Cadena Única/genética
