Abstract Polymerase chain reaction (PCR) assays targeting 16S rRNA genes followed by DNA sequencing are still important tools to characterize microbial communities present in environmental samples. However, despite the crescent number of deposited archaeal DNA sequences in databases, until now we do not ...
BACKGROUND: Liriodendron chinense ranges widely in subtropical China and northern Vietnam; however, it inhabits several small, isolated populations and is now an endangered species due to its limited seed production. The objective of this study was to develop a set of nuclear SSR (simple sequence repeats...
Li, Chao;
Fu, Gongyu;
Shi, Yaoqiang;
Zhang, A-Mei;
Xia, Xueshan;
Fang, Yue;
Mao, Xiaoqin;
Jiang, Jie;
Song, Yuzhu;
Yang, Guangying.
Klebsiella pneumoniae is one of the main pathogenic bacteria that causes nosocomial infections, such as pneumonia, urinary tract infection, and sepsis. Therefore, the rapid and accurate detection of K. pneumoniae is important for the timely treatment of infectious patients. This study aimed to establish ...
Cartilla de ADN/genética,
Cartilla de ADN/aislamiento & purificación,
Genes Bacterianos,
Klebsiella pneumoniae/genética,
Klebsiella pneumoniae/aislamiento & purificación,
Límite de Detección,
Técnicas de Amplificación de Ácido Nucleico/métodos,
Plásmidos/genética,
Plásmidos/aislamiento & purificación,
Reacción en Cadena de la Polimerasa/métodos,
Reproducibilidad de los Resultados,
Análisis de Secuencia de ADN,
Temperatura,
Factores de Tiempo
Abstract Wolbachia (Hertig) endosymbionts are extensively studied in a wide range of organisms and are known to be transmitted through the egg cytoplasm to the offsping. Wolbachia may cause several types of reproductive modifications in arthropods. In Trichogramma species, parthenogenesis-inducing Wolbac...
Alphaproteobacteria/metabolismo,
Proteínas de la Membrana Bacteriana Externa/metabolismo,
Cartilla de ADN/genética,
Genes Bacterianos/genética,
Filogenia,
Reproducción,
Especificidad de la Especie,
Simbiosis,
Avispas/genética,
Avispas/microbiología,
Wolbachia/genética
ABSTRACT We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity...
Cartilla de ADN/genética,
ADN Bacteriano/química,
ADN Bacteriano/genética,
ADN Bacteriano/aislamiento & purificación,
Separación Inmunomagnética,
Magnetismo/instrumentación,
Magnetismo/métodos,
Técnicas de Amplificación de Ácido Nucleico/instrumentación,
Técnicas de Amplificación de Ácido Nucleico/métodos,
Peste/microbiología,
Reacción en Cadena de la Polimerasa,
Sensibilidad y Especificidad,
Yersinia pestis/clasificación,
Yersinia pestis/genética,
Yersinia pestis/aislamiento & purificación
ABSTRACT Chromosome-specific probes have been widely used in molecular cytogenetics, being obtained with different methods. In this study, a reproducible protocol for construction of chromosome-specific probes is proposed which associates in situ amplification (PRINS), micromanipulation and degenerate ol...
ABSTRACT Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization of Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 strains were identified as Xanthomonas euvesicatoria and 3...
Bulgaria,
Capsicum/microbiología,
Cartilla de ADN/genética,
Variación Genética,
Grecia,
Filogenia,
Enfermedades de las Plantas/microbiología,
Reacción en Cadena de la Polimerasa,
Xanthomonas/clasificación,
Xanthomonas/genética,
Xanthomonas/aislamiento & purificación,
Xanthomonas/fisiología
Abstract Small ruminant lentiviruses (SRLV) have high genetic variability which results in different viral strains around the world. This create a challenge to design sensible primers for molecular diagnosis in different regions. This work proposes a protocol of duplex nested-PCR for the precise diagnosi...
Cartilla de ADN/genética,
ADN Viral/genética,
Enfermedades de las Cabras/diagnóstico,
Enfermedades de las Cabras/virología,
Cabras,
Infecciones por Lentivirus/diagnóstico,
Infecciones por Lentivirus/veterinaria,
Infecciones por Lentivirus/virología,
Reacción en Cadena de la Polimerasa/métodos,
Ovinos,
Enfermedades de las Ovejas/diagnóstico,
Enfermedades de las Ovejas/virología
BACKGROUND The genus Flavivirus includes a variety of medically important viruses, including dengue virus (DENV) and Zika virus (ZIKV), which are most prevalent in Brazil. Because the clinical profile of patients affected by different DENV serotypes or ZIKV may be similar, the development of new methods...
Histoplasmosis is a systemic mycosis infection caused by Histoplasma capsulatum, a heterothallic ascomycete. The sexual reproduction of this fungus is regulated by the mating type (MAT1) locus that contains MAT1-1 and MAT1-2 idiomorphs, which were identified by uniplex polymerase chain reaction (PCR). Th...
