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Knockout of caspase-7 gene improves the expression of recombinant protein in CHO cell line through the cell cycle arrest in G2/M phase

Safari, Fatemeh; Akbari, Bahman.
Biol. Res; 55 (), 2022
BACKGROUND: Chinese hamster ovary cell line has been used routinely as a bioproduction factory of numerous biopharmaceuticals. So far, various engineering strategies have been recruited to improve the production efficiency of this cell line such as apoptosis engineering. Previously, it is reported that t...
Células CHO, Caspasa 7/genética, Puntos de Control del Ciclo Celular, Proteínas Recombinantes/biosíntesis, Proteínas Recombinantes/genética, División Celular, Cricetinae, Cricetulus, Técnicas de Inactivación de Genes
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Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability

Safari, Fatemeh; Farajnia, Safar; Behbahani, Abbas Behzad; Zarredar, Habib; Barekati-Mowahed, Mazyar; Dehghani, Hesam.
Biol. Res; 53 (), 2020
BACKGROUND: Chinese hamster ovary (CHO) cells are the most commonly used mammalian host cell In the commercial-scale production of biopharmaceutical proteins. Modification of genes involved in apoptosis may improve the productivity of CHO cells. Executive caspases, including caspases 3 and 7, play critic...
Caspasa 7/deficiencia, Apoptosis, Proliferación Celular, Supervivencia Celular, Células CHO, Caspasa 7/genética, Cricetinae, Cricetulus
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