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Cloning and characterisation of four catA genes located on the chromosome and large plasmid of Pseudomonas putida ND6

Li, Shanshan; Qin, Kun; Li, Huaying; Guo, Jin; Li, Dejin; Liu, Fang; Tan, Zhilei; Yan, Wei; Qu, Shuling; Zhao, Huabing.
Electron. j. biotechnol; 34 (), 2018
Background: Although the functional redundancy of catechol 1,2-dioxygenase (C12O) genes has been reported in several microorganisms, limited enzymes were characterised, let alone the advantage of the coexistence of the multiple copies of C12O genes. Results: In this study, four novel C12O genes, designat...
Pseudomonas putida/genética, Pseudomonas putida/enzimología, Catecol 1,2-Dioxigenasa/genética, Catecol 1,2-Dioxigenasa/metabolismo, Biodegradación Ambiental, Clonación Molecular, Isoenzimas, Genes Bacterianos, Catecol 1,2-Dioxigenasa/análisis, Concentración de Iones de Hidrógeno, Temperatura, Metales
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Enhancement of biodegradation potential of catechol 1, 2-dioxygenase through its immobilization in calcium alginate gel

Guzik, Urszula; Hupert-Kocurek, Katarzyna; Marchlewicz, Ariel; Wojcieszynska, Danuta.
Electron. j. biotechnol; 17 (2), 2014
Background In biodegradation processes free enzymes often undergo deactivation. Thus, it is very important to obtain highly stable enzymes by different methods. Immobilization allows for successful stabilization of many multimeric enzymes by increasing the rigidity of the enzyme structure. This study aim...
Stenotrophomonas maltophilia, Enzimas Inmovilizadas, Catecol 1,2-Dioxigenasa/metabolismo, Biodegradación Ambiental, Alginatos, Geles, Técnicas de Cultivo de Célula, Concentración de Iones de Hidrógeno, Temperatura, Especificidad por Sustrato, Cinética
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