LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Establishment of deer heart identification method and development of the detection kit based on mitochondrial cytochrome B gene

Wang, Yanshuang; Zhang, Lihua; Ai, Jinxia.

Electron. j. biotechnol; 66 (), 2023

BACKGROUND Deer heart is a valuable traditional Chinese medicinal material. At present, there is no functional component or symbol component for deer heart, and it is difficult to identify them by physical and chemical methods. In this study, we established deer heart identification method based on mtDNA...

Ciervos/anatomía & histología, Ciervos/genética, Corazón/anatomía & histología, ADN/genética, Modelos Moleculares, Citocromos b/genética, Medicina Tradicional China

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Development of a species-specific PCR assay for authentication of Agkistrodon acutus based on mitochondrial cytochrome b gene

Li, Yingnuo; Yanshuang, Wang; Mingcheng, Li; Lihua, Zhang; Guang Xin, Yuan.

Electron J Biotechnol; 49 (), 2021

BACKGROUND: Agkistrodon acutus, a traditional Chinese medicine, clinically used in the treatment of rheumatism, tumor, and cardiovascular and cerebrovascular diseases. Due to the unique medicinal value and the difficulty of artificial breeding of Agkistrodon acutus, the supply of Agkistrodon acutus on th...

Agkistrodon/genética, Citocromos b/genética, Mitocondrias/genética, Reacción en Cadena de la Polimerasa/métodos, Medicina Tradicional China, Especificidad de la Especie, ADN/análisis, Serpientes, Clonación Molecular

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Evaluation of cytochrome b sequence to identify Leishmania species and variants: the case of Panama

Davila, Michelle; Pineda, Vanessa; Calzada, José E; Saldaña, Azael; Samudio, Franklyn.

Mem. Inst. Oswaldo Cruz; 116 (), 2021

BACKGROUND The genetic heterogeneity of Leishmania parasites is a major factor responsible for the wide variety of Leishmania-associated manifestations. Consequently, understanding the genetic make-up of Leishmania species using suitable molecular markers is an important component of realising local and...

Citocromos b/genética, ADN Protozoario/genética, Leishmania/genética, Leishmaniasis Cutánea, Panamá, Reacción en Cadena de la Polimerasa, Polimorfismo de Longitud del Fragmento de Restricción

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Morphological and molecular characterization of Haemoproteus coatneyi and Haemoproteus erythrogravidus (Haemosporida: Haemoproteidae) in Passeriformes in Brazils Atlantic Forest

Oliveira, Luísa de; Barino, Glauber Thiago Martins; Rossi, Mariana Fonseca; DAgosto, Marta; Dias, Roberto Júnio Pedroso; Santos, Huarrisson Azevedo.

Rev. bras. parasitol. vet; 29 (4), 2020

Abstract Haemoproteus spp. are protozoan parasites found in birds around the world. These parasites are identified through the morphology of gametocytes, phylogenetic analysis based on the mitochondrial cytb gene, and the parasite's geographic distribution. The absence of erythrocytic merogony, high intr...

Enfermedades de las Aves/epidemiología, Enfermedades de las Aves/parasitología, Brasil, Citocromos b/genética, Bosques, Haemosporida/clasificación, Haemosporida/genética, Passeriformes/parasitología, Filogenia, Infecciones Protozoarias en Animales/epidemiología

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