Artemisia is one of the biggest genera in the family Asteraceae, with around 500-600 taxa at specific and subspecific levels and organised in 5 subgenera. Due to the high number of taxa, a lot taxonomists are trying to solve the problem of its classification and phylogeny but its natural classification s...
BACKGROUND: Designed mimetic molecules are attractive tools in biopharmaceuticals and synthetic biology. They require mass and functional production for the assessment of upcoming challenges in the near future. The DARPin family is considered a mimetic pharmaceutical peptide group with high affinity bind...
Chloroplast biotechnology has emerged as a promissory platform for the development of modified plants to express products aimed mainly at the pharmaceutical, agricultural, and energy industries. This technology’s high value is due to its high capacity for the mass production of proteins. Moreover, the ...
In the Amazon region, agroforestry systems (AFSs) are recommended as a sustainable production alternative for local communities. A common component in Amazonian AFSs are tropical fruit trees, which can form the canopy or grow in the understory. In this study, we evaluated the effect of high radiation on ...
BACKGROUND:
Maize is one of the most important crops worldwide and has been a target of nuclear-based transformation biotechnology to improve it and satisfy the food demand of the ever-growing global population. However, the maize plastid transformation has not been accomplished due to the recalcitrant c...
Zea mays/genética,
Cloroplastos/genética,
Cloroplastos/metabolismo,
Proteínas Fluorescentes Verdes/metabolismo,
Nicotiana/metabolismo,
Proteínas Fluorescentes Verdes/genética,
Plastidios/genética,
Plantas Modificadas Genéticamente,
Biotecnología,
Transformación Genética,
Reacción en Cadena de la Polimerasa,
Escherichia coli,
Genoma del Cloroplasto
Abstract Golden trumpet, Tabebuia chrysotricha, is a native tree from the Brazilian Atlantic rain forest, with a broad latitudinal distribution. In this study, we investigated the potential effects of short-term changes in micro-weather conditions on structural features, and total protein and carbohydrat...
Background The high capacity of chloroplast genome response to integrate and express transgenes at high levels makes this technology a good option to produce proteins of interest. This report presents the stable expression of Pectin lyase (PelA gene) and the first stable expression of manganese peroxidas...
Poligalacturonasa/metabolismo,
Poligalacturonasa/genética,
Peroxidasa/metabolismo,
Peroxidasa/genética,
Cloroplastos/genética,
Nicotiana,
Hongos/enzimología,
Bacterias/enzimología,
Pared Celular,
Transformación Genética,
Hidrolasas,
Reacción en Cadena de la Polimerasa,
Temperatura,
Concentración de Iones de Hidrógeno,
Southern Blotting
Muzaffar, Adnan;
Kiani, Sarfraz;
Khan, Muhammad Azmat Ullah;
Rao, Abdul Qayyum;
Ali, Arfan;
Awan, Mudassar Fareed;
Iqbal, Adnan;
Nasir, Idrees Ahmad;
Shahid, Ahmad Ali;
Husnain, Tayyab.
BACKGROUND: Insects have developed resistance against Bt-transgenic plants. A multi-barrier defense system to weaken their resistance development is now necessary. One such approach is to use fusion protein genes to increase resistance in plants by introducing more Bt genes in combination. The locating t...
Proteínas Bacterianas/genética,
Cloroplastos/genética,
Endotoxinas/genética,
Gossypium/genética,
Proteínas Hemolisinas/genética,
Control de Insectos/métodos,
Lepidópteros,
Proteínas Recombinantes de Fusión,
Proteínas Bacterianas/análisis,
Cloroplastos/metabolismo,
Endotoxinas/análisis,
Expresión Génica/genética,
Proteínas Hemolisinas/análisis,
Resistencia a los Insecticidas/genética,
Hojas de la Planta/genética,
Transgenes/fisiología,
Bacillus thuringiensis,
Clonación Molecular,
Cartilla de ADN,
Fusión Génica,
Inmunohistoquímica,
Insecticidas,
Larva,
Microscopía de Contraste de Fase,
Plantas Modificadas Genéticamente,
Reacción en Cadena de la Polimerasa
Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pat...
Jatropha/enzimología,
Isomerasas de Doble Vínculo Carbono-Carbono/metabolismo,
Isomerasas de Doble Vínculo Carbono-Carbono/genética,
Hemiterpenos/metabolismo,
Hemiterpenos/genética,
Southern Blotting,
Clonación Molecular,
Jatropha/química,
ADN Complementario/síntesis química,
ARN/aislamiento & purificación,
Filogenia,
Reacción en Cadena de la Polimerasa de Transcriptasa Inversa,
Expresión Génica,
Cloroplastos
Background: Transgenic plants inhabiting single Bt gene are prone to develop insect resistance and this resistance has been reported in case of some important yield-devastating insect larvae of commercial crops, such as cotton and rice. Therefore, it has become essential to adapt new strategies to overco...
Cloroplastos/genética,
Cloroplastos/metabolismo,
Clonación Molecular,
Señales de Clasificación de Proteína,
Proteínas de Cloroplastos/aislamiento & purificación,
Microscopía Confocal,
Ricina/análisis,
Western Blotting,
Proteínas de Cloroplastos/genética,
Agrobacterium,
Plantas Modificadas Genéticamente,
Insectos
