Interferon-β-1a (INF-β-1a) has gained significant attention due to its emerging applications in the treatment of different human diseases. Therefore, many researchers have attempted to produce it in large quantities and also in a biologically active form using different expression systems. In the prese...
BACKGROUND: Phospholipase D (PLD) is used as the biocatalyst for phosphatidylserine (PS) production. In general, PLD was expressed in insoluble form in Escherichia coli. High-level soluble expression of PLD with high activity in E. coli is very important for industrial production of PLD. RESULTS: Strepto...
O tumor desmoide (TD) é uma proliferação fibroblástica clonal rara, caracterizada por crescimento infiltrativo e tendência à recidiva local, embora incapaz de metastatizar. A maioria desses tumores é esporádica e contém mutações no gene que codifica a proteína betacatenina. Três mutações e...
Freire, Caio César de Melo;
Palmisano, Giuseppe;
Braconi, Carla T;
Cugola, Fernanda R;
Russo, Fabiele B;
Beltrão-Braga, Patricia CB;
Iamarino, Atila;
Lima Neto, Daniel Ferreira de;
Sall, Amadou Alpha;
Rosa-Fernandes, Livia;
Larsen, Martin R;
Zanotto, Paolo Marinho de Andrade.
BACKGROUND Zika virus (ZIKV) was recognised as a zoonotic pathogen in Africa and southeastern Asia. Human infections were infrequently reported until 2007, when the first known epidemic occurred in Micronesia. After 2013, the Asian lineage of ZIKV spread along the Pacific Islands and Americas, causing s...
ABSTRACT Objective: To evaluate the association between p53 polymorphisms and human papillomavirus (HPV) E6/E7 mRNA expression. Methods: We analyzed 175 cervical samples from women aged 16-69 years old who were tested for HPV E6/E7 mRNA expression (NucliSENS® EasyQ® HPV). The samples were divided int...
Arginina/genética,
31574/virología,
Codón,
Genotipo,
Proteínas Oncogénicas Virales/genética,
Papillomaviridae/genética,
Proteínas E7 de Papillomavirus/genética,
Infecciones por Papillomavirus/virología,
Reacción en Cadena de la Polimerasa,
Polimorfismo de Longitud del Fragmento de Restricción,
ARN Mensajero/metabolismo,
ARN Viral,
Proteína p53 Supresora de Tumor/genética,
Neoplasias del Cuello Uterino/virología
ABSTRACT Cichlid fishes are an important group in evolutionary biology due to their fast speciation. This group depends widely of vision for feeding and reproduction. During the evolutionary process it plays a significant role in interspecific and intraspecific recognition and in its ecology. The molecul...
África,
Brasil,
Cíclidos/clasificación,
Cíclidos/genética,
Codón/genética,
Variación Genética,
Filogenia,
Reacción en Cadena de la Polimerasa,
Opsinas de Bastones/genética,
Análisis de Secuencia de ADN,
Especificidad de la Especie
ABSTRACT Objectives The aim of this study was to assess the possible role of HPV in the development of prostate cancer (PCa) and investigate the distribution of the p53 codon 72 polymorphism in PCa in a Turkish population. Materials and methods A total of 96 tissues, which had been obtained using a r...
Codón/genética,
ADN Viral,
Estudios de Asociación Genética,
Genotipo,
Técnicas de Genotipaje,
Persona de Mediana Edad,
Clasificación del Tumor,
Papillomaviridae/aislamiento & purificación,
Infecciones por Papillomavirus/complicaciones,
Adhesión en Parafina,
Polimorfismo Genético,
Prolina/genética,
Prostatectomía,
Hiperplasia Prostática/genética,
Hiperplasia Prostática/patología,
Hiperplasia Prostática/virología,
Neoplasias de la Próstata/genética,
Neoplasias de la Próstata/patología,
Neoplasias de la Próstata/cirugía,
Neoplasias de la Próstata/virología,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Estudios Retrospectivos,
Factores de Riesgo,
Proteína p53 Supresora de Tumor/genética,
Turquía
Background: A reliable method to detect gene polymorphisms must be established to eliminate genotyping errors due to false PCR amplification. In the previous study, we have developed AS-PCR (Allele Specific-Polymerase Chain Reaction) to detect HER2 Ile655Val gene polymorphism with good specificity and se...
Background: 3-Ketosteroid-∆¹-dehydrogenase (KSDD), a flavoprotein enzyme, catalyzes the bioconversion of 4-androstene-3,17-dione (AD) to androst-1,4-diene-3,17-dione (ADD). To date, there has been no report about characterization of KSDD from Mycobacterium neoaurum strains, which were usually empl...
CONTEXT: We verified molecular alterations in a 72-year-old Brazilian male patient with a clinical course of homozygous beta-thalassemia intermedia, who had undergone splenectomy and was surviving without regular blood transfusions. The blood cell count revealed microcytic and hypochromic anemia (hemoglo...
