Results: 19

Modified Goldner Trichrome for Non-decalcified Mineralized Tissue Plastinated and Embedded in Resin

Int. j. morphol; 42 (2), 2024
SUMMARY: Over time, Goldner's trichrome staining has been essential in paraffin soft tissue research. However, its classic application involves prior decalcification, generating disadvantages in the integrity of the samples and the interpretation of results. This study seeks to overcome the limitations a...

Identification of new human mesenchymal stem cell biomarker by aptamers

Stem cells are undifferentiated cells that can be distinguished from others by their ability to self-renew and to differentiate into new specific cell types. Mesenchymal stem cells (MSC) are adult stem cells that can be obtained from different sources, such as adipose tissue, bone marrow, dental pulp, an...

Investigation of teratogenic effects of letrozole on fetal bone development

Int. j. morphol; 40 (4), 2022
SUMMARY: Letrozole is mainly used for the treatment of unexplained infertility, breast cancer and polycystic ovarian syndrome, with secondary use in ovarian stimulation. In cases of unexpected or unknown pregnancy during the use of letrozole, letrozole may cause a teratogenic effect on the fetus. In this...

Using histological staining techniques to improve visualization and interpretability of tooth cementum annulation analysis

Int. j. morphol; 39 (1), 2021
SUMMARY: The aim of the present study was to evaluate the effect of different staining techniques on applicability and accuracy of tooth cementum annulation (TCA) method. Nine decalcination techniques, 8 dehydration protocols and 8 different techniques were applied in 3 teeth from the persons of a known ...

Value of the toluidine blue test as an aid to determine the biopsy site in actinic prurigo cheilitis

Autops. Case Rep; 11 (), 2021
Actinic prurigo (AP) is a type of photodermatosis that primarily affects the Latin American mestizo population. Histologically, AP cheilitis exhibits acanthosis with spongiosis and vacuolation of the basal cell layer overlying a dense lymphocytic inflammatory infiltrate that forms well-defined lymphoid f...

Histological comparison of two special methods of staining melanin in human skin

Int. j. morphol; 38 (6), 2020
SUMMARY: Human skin melanin was stained using the Fontana's silver nitrate method and Schmorl method. The results showed that, in the Fontana's silver nitrate method, melanin and silver-bound cells were black and other tissues were red. When stained using the Schmorl method, effects on melanin differed b...

The modified method of luxol fast blue for paraffin-embedded myelin sheath staining

Int. j. morphol; 38 (5), 2020
SUMMARY: Histological techniques are the study of animal and human tissues through staining and examining them under a microscope. To demonstrate the axonal degeneration and demyelination in histological studies, the Luxol Fast Blue staining is gold standard techniques. In this study, a new histochemical...

Morphological relationship between the superficial cortical and deep grey matter structures in adult human brains: a cadaveric study

Int. j. morphol; 37 (4), 2019
While various neurodegenerative diseases affect cortical mass differently, finding an optimal and accurate method for measuring the thickness and surface area of cerebral cortex remains a challenging problem due to highly convoluted surface of the cortex. We therefore investigated cortical thickness in a...

Evaluation of dermal collagen stained with picrosirius red and examined under polarized light microscopy

An. bras. dermatol; 93 (3), 2018
Abstract: The special picrosirius red staining highlights the natural birefringence of collagen fibers when exposed to polarized light. The results from birefringence allow to evaluate the organization of the collagen fibers in the tissues. The authors intend to elucidate all steps to obtain and capture ...