Hussain, S;
Li, X;
Bukhari, S M;
Zhou, M;
Ahmad, S;
Ahmad, S;
Javid, A;
Guan, C;
Hussain, A;
Ali, W;
Khalid, N;
Ahmad, U;
Tian, L;
Hou, Z.
Abstract The protozoans include many intracellular human pathogens. Accurate detection of these pathogens is necessary to treat the diseases. In clinical epidemiology, molecular identification of protozoan is considered a more reliable and rapid method for identification than microscopy. Among these prot...
Abstract The aim of this study was to validate a one-tube nested real-time PCR assay followed by genetic sequencing to detect and identify Cryptosporidium species and genotypes in birds. A total of 443 genomic DNA extracted from avian fecal samples were analyzed by one-tube nested real-time PCR and conve...
Abstract The consumption of vegetables has increased in recent years due to the search for a healthier diet that is rich in fiber and has fewer calories. To assess the parasitic contamination of lettuce sold in markets, a survey of parasites was carried out from a supermarket chain in the city of Londrin...
Abstract Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David's deer. In this study, 137 fecal samples from Père David's deer were collected between July 2017 and ...
China/epidemiología,
Criptosporidiosis/epidemiología,
Criptosporidiosis/parasitología,
Cryptosporidium/genética,
Cryptosporidium/aislamiento & purificación,
ADN Protozoario/genética,
Ciervos/parasitología,
Heces/parasitología,
Genotipo,
Epidemiología Molecular,
Filogenia,
Prevalencia,
ARN Ribosómico,
Análisis de Secuencia de ADN
Abstract Cryptosporidium and Giardia are protozoan parasites that cause diarrhea in humans and animals. Molecular characterization of these pathogens in sewage may provide insight on their occurrence and prevalence in Brazil. This study aimed to investigate the presence of Giardia and Cryptosporidium in ...
Ferreira, Fernanda Pinto;
Caldart, Eloiza Teles;
Freire, Roberta Lemos;
Mitsuka-Breganó, Regina;
Freitas, Felipe Machado de;
Miura, Ana Carolina;
Mareze, Marcelle;
Martins, Felippe Danyel Cardoso;
Urbano, Mariana Ragassi;
Seifert, Adilson Luiz;
Navarro, Italmar Teodorico.
Abstract The objective of this study was to determine factors associated with vegetable contamination with zoonotic protozoan. Samples of water, soil and vegetables were collected from July/2014 to May/2016, totaling 83 samples, 21 properties of Londrina region, Paraná, Brazil. DNA amplification of Tox...
Cryptosporidium/genética,
Cryptosporidium/aislamiento & purificación,
ADN Protozoario/genética,
Giardia lamblia/genética,
Giardia lamblia/aislamiento & purificación,
Reacción en Cadena de la Polimerasa,
Suelo/parasitología,
Toxoplasma/genética,
Toxoplasma/aislamiento & purificación,
Verduras/parasitología,
Agua/parasitología
ABSTRACT The study was conducted to compare the specificity of immunological diagnostic methods used for the diagnosis of Cryptosporidium species capable of causing life-threatening infection in both immunosuppressed and immunocompetent patients. For the detection of Cryptosporidium species in 79 animals...
Criptosporidiosis/diagnóstico,
Criptosporidiosis/microbiología,
Cryptosporidium/genética,
Cryptosporidium/inmunología,
Cryptosporidium/aislamiento & purificación,
Diarrea/diagnóstico,
Diarrea/microbiología,
Diarrea/veterinaria,
Pruebas Inmunológicas/economía,
Pruebas Inmunológicas/métodos,
Pruebas Inmunológicas/veterinaria,
Sensibilidad y Especificidad
Abstract This study used several diagnostic methods to examine the occurrence of and molecularly characterize Cryptosporidium spp. in captive canaries (Serinus canaria) in southern and southeastern Brazil. A total of 498 fecal samples were purified by centrifugal-flotation using Sheather's solution. Cryp...
Ewald, Maria Paula de Carvalho;
Martins, Felippe Danyel Cardoso;
Caldart, Eloiza Teles;
Vieira, Fernando Emmanuel Gonçalves;
Yamamura, Milton Hissashi;
Sasse, João Pedro;
Barros, Luiz Daniel de;
Freire, Roberta Lemos;
Navarro, Italmar Teodorico;
Garcia, João Luis.
Abstract Rearing free-range chicken is based on grazing feeding patterns, and these animals could be potential environmental contaminants of Cryptosporidium oocysts for humans and other animals. Therefore, the present study aimed to evaluate the molecular prevalence of Cryptosporidium spp. in free-range ...
As doenças de veiculação hídrica, sobretudo aquelas causadas por protozoários intestinais, emergiram como um dos principais problemas de saúde pública. Diferentes aspectos são abordados sobre a biologia e a epidemiologia dos principais protozoários parasitas de transmissão hídrica. Cryptospori...
