LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Transcriptome differences between 20- and 3, 000-year-old Platycladus orientalis reveal that ROS are involved in senescence regulation

Chang, Ermei; Zhang, Jing; Deng, Nan; Yao, Xiamei; Liu, Jianfeng; Zhao, Xiulian; Jiang, Zeping; Shi, Shengqing.

Electron. j. biotechnol; 29 (), 2017

Background: Platycladus orientalis has an extremely long life span of several thousands of years, attracting great interests in the mechanisms involved in such successful senescence regulation and resistance at physiological and molecular levels. Results: The levels of reactive oxygen species (ROS) were ...

Envejecimiento/genética, Cupressaceae/genética, Especies Reactivas de Oxígeno, Transcriptoma, Regulación de la Expresión Génica, Análisis de Secuencia de ARN, Homeostasis, Depuradores de Radicales Libres, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa

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Isolation of high-quality RNA from Platycladus orientalis and other Cupressaceae plants

Chang, Ermei; Zhao, Yuxin; Wei, Qianchun; Shi, Shengqing; Jiang, Zeping.

Electron. j. biotechnol; 19 (5), 2016

Platycladus orientalis has a lifespan of several thousand years in China, making it a good plant in which to study aging at the molecular level, but this requires sufficient quantities of high-quality P. orientalis RNA. However, no appropriate methods have been reported for total RNA isolation from P. or...

Cupressaceae/genética, ARN/aislamiento & purificación, Polifenoles, Hojas de la Planta/genética, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa, Dodecil Sulfato de Sodio, Secuenciación del Exoma, Perfilación de la Expresión Génica, Polisacáridos

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DNA sequence variation of drought-response candidate genes in Austrocedrus chilensis

Pomponio, María F; Torales, Susana; Gallo, Leonardo A; Pastorino, Mario J; Marchelli, Paula; Cervera, María Teresa; Poltri, Susana Marcucci.

Electron. j. biotechnol; 16 (2), 2013

Background: Austrocedrus chilensis (D. Don) Pic. Ser. et Bizzarri commonly known as Patagonian cypress is a member of the Cupressaceae family, characterized by a high adaptive potential for growing in marginal areas and good timber quality. The species grows over a wide area and under a wide range of rai...

Cupressaceae/genética, Cupressaceae/fisiología, Sequías, Adaptación Fisiológica/genética, Secuencia de Bases, Variación Genética, Polimorfismo de Nucleótido Simple, Selección Genética, Reacción en Cadena de la Polimerasa, Estructuras Genéticas, Genética de Población, Nucleótidos/genética

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