Results: 10

Role of extracellular DNA in Enterococcus faecalis biofilm formation and its susceptibility to sodium hypochlorite

J. appl. oral sci; 27 (), 2019
Abstract Objective This study investigated the role of extracellular deoxyribonucleic acid (eDNA) on Enterococcus faecalis ( E. faecalis ) biofilm and the susceptibility of E. faecalis to sodium hypochlorite (NaOCl). Methodology E. faecalis biofilm was formed in bovine tooth specimens and the biofilm ...

O papel das nucleases no laboratório de biologia molecular: vilãs ou aliadas?
The role of nucleases in the molecular biology laboratory: villains or allies?

Em laboratório de biologia molecular existem normas para prevenir que nucleases destruam os ácidos nucleicos em análise. Rígida adesão a estas normas é primordial, principalmente em laboratórios de análises clínicas e ao se lidar com amostras com número restrito de cópias do genoma-alvo. Em co...

Enzyme activity and thermostability of a non-specific nuclease from Yersinia enterocolitica subsp. palearctica by site-directed mutagenesis

Electron. j. biotechnol; 19 (6), 2016
Background: To identify the critical amino acid residues that contribute to the high enzyme activity and good thermostability of Yersinia enterocolitica subsp. palearctica (Y. NSN), 15 mutants of Y. NSN were obtained by site-directed mutagenesis in this study. And their enzyme activity and thermostabilit...

Functional disruption of human leukocyte antigen II in human embryonic stem cell

Biol. Res; 48 (), 2015
BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact sam...

Teste de DNA para verificaçäo de parentesco em cäes: avaliaçäo do método näo automatizado com o auxílio do primer CMR S
DNA test for parentage verification in dogs: evaluation of the non-automatized method with assistance of the primer CMR S

To evaluate the precision of the DNA tests using the non-automatized technique for individual identification and parentage tests, 105 Rottweiler dogs were studied using the primer CMR S. The sample was composed of 39 animals belonging to 11 complete families and their progenies, and 66 non related indivi...

Deteccion de la Desoxirribonucleasa Termoestable (Terminucleasa) directamente en el alimento
Detection of deoxyribonuclease thermostable (thermonuclease) directly in food thermostble

Rev. cuba. aliment. nutr; 10 (2), 1996
Se inocularon in vitro diversos alimentos de origen animal, con la cepa de Staphylococcus aureus FRI-S6, productora de la enterotoxina estafilococica del serotipo B y de la nucleasa tersmoestable. Se comprobo la concentracion microbiana de los inoculos, mediante siembra en superficie en medio Baird Parke...

Uma nova associaçäo antimicrobiana no desbridamento enzimático com colagenase
A new antimicrobial association in the enzymatic debridment with collagenase

Rev. bras. cir; 82 (5), 1992
A substituiçao do Cloranfenicol pela Gentamicina, associada à colagenase Clostridium peptidase A mostrou-se vantajosa na eliminaçao da escara em 66 doentes portadores de lesoes causadas por patologias diversas. Esta eliminaçao foi mais eficaz por sua precocidade, homogeneidade, facilidade e sobretudo...

Tratamento topico das ulceras venosas com uma associacao de fibrinolisina, desoxirribonuclease e cloranfenicol.
Topic treatment of venous ulcers with an association of fibrinolysin, desoxyribonuclease and chloramphenicol

Rev. bras. cir; 72 (3), 1982
Realizou-se um estudo clinico aberto, nao controlado, em pacientes com ulceras venosas, nos quais se aplicou topicamente uma associacao de fibrinolisina, desoxirribonuclease e cloranfenicol, duas vezes por dia. Avaliou-se o grau de granulacao e cicatrizacao, a limpeza da lesao, o desaparecimento dos sina...