Results: 3

Determination of multidrug resistance mechanisms in Clostridium perfringens type A isolates using RNA sequencing and 2D-electrophoresis

Braz. j. med. biol. res; 51 (8), 2018
In this study, we screened differentially expressed genes in a multidrug-resistant isolate strain of Clostridium perfringens by RNA sequencing. We also separated and identified differentially expressed proteins (DEPs) in the isolate strain by two-dimensional electrophoresis (2-DE) and mass spectrometry (...

Displacers improve the selectivity of phosphopeptide enrichment by metal oxide affinity chromatography

Abstract: Background: A key process in cell regulation is protein phosphorylation, which is catalyzed by protein kinases and phosphatases. However, phosphoproteomics studies are difficult because of the complexity of protein phosphorylation and the number of phosphorylation sites. Methods: We describ...

Interação parasita-célula hospedeira: modificação de proteínas de Tripanosoma cruzi durante adesão à matriz extracelular

A doença de Chagas foi incialmente descrita em 1090 e após mais de 100 anos de investigações sobre essa doença, ainda pouco se sabe sobre os mecanismos ativados no parasita durante sua adesão e invasão à célula hospedeira. Glicoproteínas de massa molecular de 85kDa localizadas na membrana do pa...