BACKGROUND: Lignocellulose is considered a renewable organic material, but the industrial production of biofuel from lignocellulose is challenging because of the lack of highly active hydrolytic enzymes. The guts of herbivores contain many symbiotic microorganisms that have evolved to hydrolyze plant lig...
Background: The bioethanol produced from biomass is a promising alternative fuel. The lignocellulose from marginal areas or wasteland could be a promising raw material for bioethanol production because it is present in large quantities, is cheap, renewable and has favorable environmental properties. Desp...
Background: Plastic waste is a serious problem because it is difficult to degrade, thereby leading to global environment problems. Poly(lactic acid) (PLA) is a biodegradable aliphatic polyester derived from renewable resources, and it can be degraded by various enzymes produced by microorganisms. This st...
Background: Mathematical modeling is useful in the analysis, prediction, and optimization of an enzymatic process. Unlike the conventional modeling methods, Monte Carlo method has special advantages in providing representations of the molecule’s spatial distribution. However, thus far, Monte Carlo mode...
Background: Astaxanthin from natural sources is typically esterified with fatty acids; hence, it must be hydrolyzed to remove esters before identification and quantification by conventional HPLC. Alkaline-catalyzed saponification and enzyme-catalyzed enzymolysis are the most commonly used de-esterificati...
ABSTRACT Several enzymatic reactions of heteroatom-containing compounds have been explored as unnatural substrates. Considerable advances related to the search for efficient enzymatic systems able to support a broader substrate scope with high catalytic performance are described in the literature. These ...
Background: Poly(DL-lactic acid), or PDLLA, is a biodegradable polymer that can be hydrolyzed by various types of enzymes. The protease produced by Actinomadura keratinilytica strain T16-1 was previously reported to have PDLLA depolymerase activity. However, few studies have reported on PDLLA-degrading e...
Background: Defense-related anti-oxidative response is a vital defense mechanism of plants against pathogen invasion. Ralstonia solanacearum is an important phytopathogen. Bacterial wilt caused by R. solanacearum is the most destructive disease and causes severe losses in patchouli, an important aromatic...
Pogostemon/enzimología,
Pogostemon/microbiología,
Pogostemon/inmunología,
Superóxido Dismutasa/metabolismo,
Catecol Oxidasa/metabolismo,
Ralstonia solanacearum/patogenicidad,
Virulencia,
Peroxidasa/metabolismo,
Fenilanina Amoníaco-Liasa/metabolismo,
Enzimas/metabolismo,
Electroforesis en Gel de Poliacrilamida Nativa,
Enzimas/inmunología,
Ralstonia solanacearum/fisiología,
Antioxidantes,
Electroforesis en Gel de Poliacrilamida
Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. Results: To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant...
Isomaltosa/metabolismo,
Oligosacáridos/metabolismo,
Enzimas/metabolismo,
Almidón/metabolismo,
alfa-Amilasas/metabolismo,
beta-Amilasa/metabolismo,
Glucosidasas/metabolismo,
Bacillus/enzimología,
Aspergillus niger/enzimología,
Oligosacáridos/biosíntesis,
Licuefacción,
Concentración de Iones de Hidrógeno,
Glicosilación,
Temperatura,
Glicósido Hidrolasas/metabolismo,
Fermentación
CASUISTICA E METODOS: Realizamos uma análise metabólica da cardioplegia sanguínea hipotérmica retrógrada contínua em um estudo prospectivo de 15 pacientes consecutivos encaminhados para operaçäo eletiva de revascularizaçäo miocárdica. Os critérios de inclusäo foram doença coronária bi ou t...
