LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Optimization of fructose-rich syrups production from Opuntia ficus-indica inulin using immobilized inulinase on Luffa cylindrical

Lara Fiallos, Marco; Montalvo-Villacreses, Dayana; Espín-Valladares, Rosario C; Nuñez-Pérez, Jimmy; Pérez-Martínez, Amaury; González-Suárez, Erenio; Pais-Chanfrau, José M.

Electron. j. biotechnol; 66 (), 2023

BACKGROUND Luffa cylindrica has numerous domestic and industrial uses. For example, this natural fiber supports immobilization through the covalent bonding of an inulinase. In addition, fructose syrup from crude inulin obtained from prickly pear (Opuntia ficus-indica) can be obtained in a plug-flow mini ...

Inulina/química, Inulina/metabolismo, Fructosa/análisis, Opuntia/química, Opuntia/metabolismo, Luffa/metabolismo, Luffa/enzimología, Enzimas Inmovilizadas/química, Enzimas Inmovilizadas/metabolismo

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Immobilization of cyclodextrin glucanotransferase on aminopropyl-functionalized silica-coated superparamagnetic nanoparticles

Ibrahim, Abdelnasser S. S; Al-Salamah, Ali A; El-Toni, Ahmed Mohamed; El-Tayeb, Mohamed A; Elbadawi, Yahya B.

Electron. j. biotechnol; 16 (6), 2013

Background: Cyclodextrin glycosyltransferase (CGTase) from Amphibacillus sp. NPST-10 was successfully covalently immobilized on aminopropyl-functionalized silica coated superparamagnetic nanoparticles; and the properties of immobilized enzyme were investigated. The synthesis process included preparing of...

Glucosiltransferasas/química, Enzimas Inmovilizadas/química, Nanopartículas de Magnetita/química, Dióxido de Silicio, Bacillaceae/enzimología, Técnicas de Cultivo, Glucosiltransferasas/biosíntesis, Glucosiltransferasas/aislamiento & purificación, Concentración de Iones de Hidrógeno, Temperatura, Cinética, Glucosiltransferasas/metabolismo, Enzimas Inmovilizadas/metabolismo, Ciclodextrinas, Espectrofotometría Infrarroja

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Immobilization and stability studies of a lipase from thermophilic Bacillus sp: The effect of process parameters on immobilization of enzyme

Nawani, Neerupma; Singh, Rajvinder; Kaur, Jagdeep.

Electron. j. biotechnol; 9 (5), 2006

A thermostable lipase was partially purified from the culture supernatant of a thermophilic Bacillus sp. The enzyme is optimally active at 60ºC and pH 8.0. The enzyme showed enhancement in activity in presence of benzene or hexane (30 percent v/v each). The activity (assayed by determining the release o...

Bacillus/enzimología, Enzimas Inmovilizadas/química, Esterificación, Concentración de Iones de Hidrógeno, Temperatura, Lipasa, Estabilidad de Enzimas, Semivida, Ácido Oléico, Dióxido de Silicio

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