Abstract As an important enzyme, xylanase is widely used in the food, pulp, and textile industry. Different applications of xylanase warrant specific conditions including temperature and pH. This study aimed to carry out sodium alginate beads as carrier to immobilize previous reported mutated xylanase fr...
Fosfomycin tromethamol (FT) was reintroduced as an option for the treatment of low urinary tract infection (UTI) in children. In this study, we described the antibiotic sensitivity and mechanisms of resistance to fosfomycin in isolates from children older than 6 years with UTI. Urine culture and antibiot...
Antibacterianos/farmacología,
Farmacorresistencia Bacteriana,
Escherichia coli/genética,
Infecciones por Escherichia coli/tratamiento farmacológico,
Infecciones por Escherichia coli/epidemiología,
Fosfomicina/farmacología,
Fosfomicina/uso terapéutico,
Pruebas de Sensibilidad Microbiana,
Filogenia,
Infecciones Urinarias/tratamiento farmacológico,
beta-Lactamasas/genética
Glutamine synthetase (GS), encoded by glnA, catalyzes the conversion of L-glutamate and ammonium to L-glutamine. This ATP hydrolysis driven process is the main nitrogen assimilation pathway in the nitrogen-fixing bacterium Azospirillum brasilense. The A. brasilense strain HM053 has poor GS activity and l...
Alpha amylase, catalyzing the hydrolysis of starch is a ubiquitous enzyme with tremendous industrial applications. A 1698 bp gene coding for 565 amino acid amylase was PCR amplified from Geobacillus thermodenitrificans DSM465, cloned in pET21a (+) plasmid, expressed in BL21 (DE3) strain of E. coli and ch...
L-Asparaginase catalysing the breakdown of L-Asparagine to L-Aspartate and ammonia is an enzyme of therapeutic importance in the treatment of cancer, especially the lymphomas and leukaemia. The present study describes the recombinant production, properties and anticancer potential of enzyme from a hypert...
Antineoplásicos/farmacología,
Asparaginasa/biosíntesis,
Asparaginasa/farmacología,
Células CACO-2,
Estabilidad de Enzimas,
Escherichia coli/genética,
Concentración de Iones de Hidrógeno,
Simulación del Acoplamiento Molecular,
Pyrococcus abyssi/enzimología,
Proteínas Recombinantes/biosíntesis,
Proteínas Recombinantes/farmacología,
Especificidad por Sustrato
BACKGROUND: Aerobic metabolism generates reactive oxygen species that may cause critical harm to the cell. The aim of this study is the characterization of the stress responses in the model aromatic degrading bacterium Paraburkholderia xenovorans LB400 to the oxidizing agents paraquat and H 2 O2. METHODS...
Proteínas de Escherichia coli/genética,
Proteínas de Escherichia coli/metabolismo,
Proteínas Bacterianas/genética,
Proteínas Bacterianas/metabolismo,
Escherichia coli/genética,
Regulación Bacteriana de la Expresión Génica,
Peróxido de Hidrógeno/farmacología,
Proteínas Represoras/genética,
Proteínas Represoras/metabolismo,
Espectrometría de Masas en Tándem,
Estrés Oxidativo,
Oxidación-Reducción,
Cromatografía Liquida,
Burkholderiaceae
Abstract Pathogenic strains of Escherichia coli may invade the subcutaneous tissue of poultry and cause cellulitis, whilst the pathogen may also cause lesions in internal organs such as the liver. Current paper co-relates Escherichia coli and virulence genes characteristic of Avian Pathogenic Escherichia...
Umpiérrez, Ana;
Ernst, Déborah;
Fernández, Magalí;
Oliver, Martín;
Casaux, María L.;
Caffarena, Rubén D.;
Schild, Carlos;
Giannitti, Federico;
Fraga, Martín;
Zunino, Pablo.
Enfermedades de los Bovinos,
Infecciones por Escherichia coli,
Proteínas de Escherichia coli,
Enfermedades de los Bovinos/epidemiología,
Diarrea/epidemiología,
Diarrea/veterinaria,
Escherichia coli/genética,
Infecciones por Escherichia coli/epidemiología,
Infecciones por Escherichia coli/veterinaria,
Proteínas de Escherichia coli/genética,
Heces,
Virulencia/genética,
Factores de Virulencia/genética
Feed is the main route of transmission of pathogenic microorganisms and is responsible for a large part of the cost of poultry production, so the inclusion of alternative foods in diets for monogastrics has been a constant. Among alternative foods most used in modern poultry farming are animal meal, howe...
BACKGROUND: Poly-3-hydroxybutyrate (PHB) can be efficiently produced in recombinant Escherichia coli by the overexpression of an operon (NphaCAB) encoding PHB synthetase. Strain improvement is considered to be one of critical factors to lower the production cost of PHB in recombinant system. In this stud...
Hidroxibutiratos/metabolismo,
Escherichia coli/metabolismo,
Escherichia coli/genética,
Eliminación de Gen,
Proteínas Represoras/genética,
Proteínas de Unión al ARN/genética,
Proteínas de Escherichia coli/genética,
Biopolímeros/genética,
Ligasas/metabolismo,
Proteínas Recombinantes,
Ingeniería Metabólica
