Enzymes of the archaea living in extreme environments are resistant to the challenging conditions. Lipase is among the important enzymes used in the industry and agriculture. In this study, the extracellular lipase from extremely halophilic archaeon Halolamina sp. was characterized for the first time. Op...
Enzymes immobilized onto substrates with excellent selectivity and activity show a high stability and can withstand extreme experimental conditions, and their performance has been shown to be retained after repeated uses. Applications of immobilized enzymes in various fields benefit from their unique cha...
Alpha amylase, catalyzing the hydrolysis of starch is a ubiquitous enzyme with tremendous industrial applications. A 1698 bp gene coding for 565 amino acid amylase was PCR amplified from Geobacillus thermodenitrificans DSM465, cloned in pET21a (+) plasmid, expressed in BL21 (DE3) strain of E. coli and ch...
L-Asparaginase catalysing the breakdown of L-Asparagine to L-Aspartate and ammonia is an enzyme of therapeutic importance in the treatment of cancer, especially the lymphomas and leukaemia. The present study describes the recombinant production, properties and anticancer potential of enzyme from a hypert...
Antineoplásicos/farmacología,
Asparaginasa/biosíntesis,
Asparaginasa/farmacología,
Células CACO-2,
Estabilidad de Enzimas,
Escherichia coli/genética,
Concentración de Iones de Hidrógeno,
Simulación del Acoplamiento Molecular,
Pyrococcus abyssi/enzimología,
Proteínas Recombinantes/biosíntesis,
Proteínas Recombinantes/farmacología,
Especificidad por Sustrato
Zhang, Rui;
Li, Xin-Yue;
Cen, Xiao-Long;
Gao, Qi-Hui;
Zhang, Mei;
Li, Kong-Yue;
Wu, Qian;
Mu, Yue-Lin;
Tang, Xiang-Hua;
Zhou, Jun-Pei;
Huang, Zun-Xi.
BACKGROUND: Manno-oligosaccharides (MOS) is known as a kind of prebiotics. Mannanase plays a key role for the degradation of mannan to produce MOS. In this study, the mannanases of glycoside hydrolase (GH) families 5 Man5HJ14 and GH26 ManAJB13 were employed to prepare MOS from locust bean gum (LBG) and p...
El género Pseudomonas es una fuente importante de proteasas; sin embargo, su uso está restringido en la industria alimentaria. El clonaje permite aprovechar la capacidad catalítica de estas enzimas mediante su producción en microorganismos inocuos. Por otro lado, las leguminosas son fuentes ricas en ...
Background: Textile industry not only plays a vital role in our daily life but also a prominent factor in improving global economy. One of the environmental concern is it releases huge quantities of toxic dyes in the water leading to severe environmental pollution. Bacterial laccase and azoreductase succ...
NADH NADPH Oxidorreductasas/química,
Lacasa/química,
Peroxidasa/química,
Industria Textil,
Compuestos Azo/metabolismo,
Colorantes/metabolismo,
Estabilidad de Enzimas,
Temperatura,
Simulación por Computador,
Biodegradación Ambiental,
Compuestos Azo/química,
NADH NADPH Oxidorreductasas/metabolismo,
Lactasa/metabolismo,
Peroxidasa/metabolismo
Background: The aim of this work was to purify and characterize exo-β-1,3-glucanase, namely, TtBgnA, from the thermophilic fungus Thielavia terrestris Co3Bag1 and to identify the purified enzyme. Results: The thermophilic biomass-degrading fungus T. terrestris Co3Bag1 displayed β-1,3-glucanase activity...
Sordariales/enzimología,
Glucano 1,3-beta-Glucosidasa/química,
Glucano 1,3-beta-Glucosidasa/aislamiento & purificación,
Espectrometría de Masas en Tándem,
Celulasas,
Electroforesis en Gel de Poliacrilamida,
Pruebas de Enzimas,
Concentración de Iones de Hidrógeno,
Estabilidad de Enzimas,
Temperatura
Huang, Lin;
Zheng, Dong;
Zhao, Yatong;
Ma, Jieying;
Li, Yanzhen;
Xu, Zehua;
Shan, Mengying;
Shao, Shulin;
Guo, Qingwen;
Zhang, Jie;
Lu, Fuping;
Liu, Yihan.
BACKGROUND: Lipases are extensively exploited in lots of industrial fields; cold-adapted lipases with alkali-resistance are especially desired in detergent industry. Penicillium cyclopium lipase I (PCL) might be suitable for applications of detergent industry due to its high catalytic efficiency at low t...
Penicillium/enzimología,
Estabilidad de Enzimas,
Lipasa/metabolismo,
Álcalis,
Biocatálisis,
Reacción en Cadena de la Polimerasa/métodos,
Frío,
Penicillium/genética,
Lipasa/genética,
Penicillium/aislamiento & purificación,
Lipasa/aislamiento & purificación,
Mutación,
Industria de Detergentes,
Concentración de Iones de Hidrógeno,
Interacciones Hidrofóbicas e Hidrofílicas
Background: Lactate dehydrogenase (LDH) is an enzyme of glycolytic pathway, ubiquitously found in living organisms. Increased glycolysis and LDH activity are associated with many pathologic conditions including inflammation and cancer, thereby making the enzyme a suitable drug target. Studies on conserve...
