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Effect of encephalomyocarditis virus 3'Untranslated region on GFP transient expression: implications in recombinant protein production

Marzbany, Marzieh; Rasekhian, Mahsa.
Bol. latinoam. Caribe plantas med. aromát; 19 (6), 2020
The enrichment of therapeutic protein production yield in mammalian cell cultures by modulating mRNA stability is a fairly new strategy in biotechnological applications. Here, we describe the application of 3′-untranslated region (3′UTR) from RNA viral genome to modulate mRNA stability.The data obtai...
Virus de la Encefalomiocarditis/metabolismo, Proteínas Recombinantes/biosíntesis, Regiones no Traducidas, Productos Biológicos, Biotecnología, Proteínas Fluorescentes Verdes/metabolismo, Estabilidad del ARN, Técnicas de Cultivo de Célula, Virus de la Encefalomiocarditis/genética, Genoma Viral
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Improving the expression of recombinant pullulanase by increasing mRNA stability in Escherichia coli

Li, Tao; Ding, Yatong; Zhang, Jun; Jiao, Guobao; Sun, Lipeng; Liu, Zhongmin; Qiu, Liyou.
Electron. j. biotechnol; 29 (), 2017
Background: Pullulanase production in both wild-type strains and recombinantly engineered strains remains low. The Shine-Dalgarno (SD) sequence and stem-loop structure in the 5′ or 3′ untranslated region (UTR) are well-known determinants of mRNA stability. This study investigated the effect of mRNA s...
Escherichia coli/enzimología, Escherichia coli/genética, Glicósido Hidrolasas/metabolismo, Estabilidad del ARN, Glicósido Hidrolasas/genética, Fermentación, Expresión Génica, Vectores Genéticos, Transformación Genética, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa
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