SUMMARY OBJECTIVE: The objective of this study was to explore the molecular mechanism underlying the occurrence of benign bile duct stricture and the target of low-dose paclitaxel in the prevention of benign bile duct stricture. METHODS: Under the stimulation of transforming growth factor beta 1, the e...
Cheng, Gong;
Abbas Raza, Sayed Haidar;
Khan, Rajwali;
Wang, Hong;
Shater, Abdullah F;
Mohammedsaleh, Zuhair M;
Wang, Li;
Tian, Yuan;
Long, Feng;
Zan, Linsen.
BACKGROUND: Adipogenesis and fibrogenesis can be considered as a competitive process in muscle, which may affect the intramuscular fat deposition. The CCAAT/enhancer-binding protein beta (C/EBPb) plays an important role in adipogenesis, which is well-characterized in mice, but little known in bovine so f...
Bovinos/metabolismo,
Fibroblastos/metabolismo,
Adipocitos/metabolismo,
Adipogénesis,
Tejido Adiposo/metabolismo,
Proteína beta Potenciadora de Unión a CCAAT/metabolismo,
Reacción en Cadena en Tiempo Real de la Polimerasa,
Proliferación Celular,
Mitosis,
Células Clonales,
Músculos
ABSTRACT Objective: To observe clinically, in rabbits, the side effects of topical injection of subconjunctival cyclophosphamide, studying its role as an antifibrotic drug. Methods: Prospective study in 20 albino rabbits of New Zealand race. All rabbits were treated with cyclophosphamide, 10mg/ml in a ...
Ciclofosfamida/administración & dosificación,
Ciclofosfamida/farmacología,
Fibrosis/prevención & control,
Inyecciones Intraoculares,
Conjuntiva/efectos de los fármacos,
Cicatrización de Heridas/efectos de los fármacos,
Ciclofosfamida/efectos adversos,
Microscopía con Lámpara de Hendidura,
Fibroblastos/metabolismo,
Mitomicina/farmacología,
Fibroblastos/efectos de los fármacos,
Estudios Prospectivos
The objective of this study was to investigate the effect of human esophageal fibroblast-derived exosomal miR-21 on cisplatin sensitivity against esophageal squamous EC9706 cells. EC9706 cells were co-cultured indirectly with human esophageal fibroblasts (HEF) or miR-21 mimics transfected-HEF in the tran...
Apoptosis,
Proteínas Reguladoras de la Apoptosis/metabolismo,
Carcinoma,
Línea Celular Tumoral,
Proliferación Celular,
Cisplatino/farmacología,
Neoplasias Esofágicas/tratamiento farmacológico,
Neoplasias Esofágicas/genética,
Fibroblastos/metabolismo,
MicroARNs/genética,
Proteínas de Unión al ARN,
Microambiente Tumoral
Abstract This study evaluated the effect of a cyclopentenone-type PG, 15-Deoxy-Δ12,14-PG J2 (15d-PGJ2), and lectin (ScLL) on the viability of human gingival fibroblasts (HGFs), and on IL-6 and TGFβ-1 release by these fibroblasts, stimulated with lipopolysaccharide (LPS). HGFs were stimulated wi...
Análisis de Varianza,
Supervivencia Celular/efectos de los fármacos,
Células Cultivadas,
Ensayo de Inmunoadsorción Enzimática,
Fibroblastos/efectos de los fármacos,
Fibroblastos/metabolismo,
Encía/citología,
Interleucina-6/metabolismo,
Lipopolisacáridos/farmacología,
Lectinas de Plantas/farmacología,
Prostaglandina D2/análogos & derivados,
Prostaglandina D2/farmacología,
Valores de Referencia,
Estadísticas no Paramétricas,
Factores de Tiempo,
Factor de Crecimiento Transformador beta1/efectos de los fármacos,
Factor de Crecimiento Transformador beta1/metabolismo
Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting...
Western Blotting,
Diferenciación Celular/fisiología,
Proliferación Celular/fisiología,
Fibroblastos/metabolismo,
Fibroblastos/fisiología,
Técnica del Anticuerpo Fluorescente,
Proteínas Musculares/metabolismo,
Proteínas Musculares/fisiología,
Músculo Esquelético/metabolismo,
Músculo Esquelético/fisiología,
Ubiquitina-Proteína Ligasas/metabolismo,
Ubiquitina-Proteína Ligasas/fisiología
BACKGROUND: Conjunctival filtering bleb scar formation is the main reason for the failure of glaucoma filtration surgery. Cytoglobin (Cygb) has been reported to play an important role in extracellular matrix (ECM) remodeling, fibrosis and tissue damage repairing. This study aimed to investigate the role ...
Abstract Purpose: To evaluate the efficacy of the application of the human amniotic membrane (HAM) on the inflammatory process, fibroblast proliferation, formation of collagenand reduction of skin wound areas in rats. Methods: Thirty six rats were submitted to a surgical injury induction and divided ...
Amnios/química,
Amnios/trasplante,
Apósitos Biológicos,
Colágeno/farmacología,
Colágeno Tipo I/metabolismo,
Colágeno Tipo I/farmacología,
Colágeno Tipo III/metabolismo,
Colágeno Tipo III/farmacología,
Modelos Animales de Enfermedad,
Fibroblastos/metabolismo,
Fibroblastos/patología,
Inflamación/metabolismo,
Distribución Aleatoria,
Ratas Wistar,
Piel/lesiones,
Piel/patología,
Cicatrización de Heridas/efectos de los fármacos,
Cicatrización de Heridas/fisiología
Abstract Purpose: To evaluate the changes of caveolin-1 in lung fibroblasts in newborn Wistar rats when exposed to hyperoxic conditions, as well as lung fibroblasts cell cycle. Methods: One hundred newborn Wistar rats were randomly divided (50 rats/group) into experimental and control groups, exposed ...
Caveolina 1/metabolismo,
Proliferación Celular,
Fibroblastos/metabolismo,
Enfermedades Pulmonares/metabolismo,
Pulmón/metabolismo,
Animales Recién Nacidos,
Caveolina 1/farmacología,
Ciclo Celular,
Células Cultivadas,
Enfermedad Crónica,
Fibroblastos/citología,
Fibroblastos/efectos de los fármacos,
Hiperoxia,
Pulmón/citología,
Pulmón/efectos de los fármacos,
Enfermedades Pulmonares/inducido químicamente,
Enfermedades Pulmonares/clasificación,
Modelos Animales,
Oxígeno/farmacología,
Distribución Aleatoria,
Ratas Wistar
BACKGROUND: Hypertrophic scarring (HS) is a severe disease, and results from unusual wound healing. Col1A1 could promote the hypertrophic scar formation, and the expression of Col1A1 in HS tissue was markedly higher than that in the normal. In present study, we aimed to identify miRNAs as post-transcript...
Apoptosis/genética,
Colágeno Tipo I/metabolismo,
Fibroblastos/metabolismo,
MicroARNs/genética,
Procesamiento Postranscripcional del ARN/genética,
Cicatriz Hipertrófica/genética,
Cicatriz Hipertrófica/metabolismo,
Colágeno Tipo I/genética,
MicroARNs/metabolismo,
Estudios de Casos y Controles,
Proliferación Celular,
Regulación hacia Abajo
