Displacers improve the selectivity of phosphopeptide enrichment by metal oxide affinity chromatography
Bol. méd. Hosp. Infant. Méx; 74 (3), 2017
Abstract: Background: A key process in cell regulation is protein phosphorylation, which is catalyzed by protein kinases and phosphatases. However, phosphoproteomics studies are difficult because of the complexity of protein phosphorylation and the number of phosphorylation sites. Methods: We describ...
Cromatografía de AfinidadMétodos, Fosfopéptidos/análisis, Fosfoproteínas/análisis, Titanio/química, Electroforesis en Gel BidimensionalMétodos, Phaseolus/metabolismo, Fosforilación, Rhizobium/metabolismo, Espectrometría de Masa por Láser de Matriz Asistida de Ionización DesorciónMétodos, Simbiosis/fisiología