Application of n-dodecane as an oxygen vector to enhance the activity of fumarase in recombinant Escherichia coli: role of intracellular microenvironment
Braz. j. microbiol; 49 (3), 2018
Abstract The effect of the intracellular microenvironment in the presence of an oxygen vector during expression of a fusion protein in Escherichia coli was studied. Three organic solutions at different concentration were chosen as oxygen vectors for fumarase expression. The addition of n-dodecane did not...
Alcanos/química, Alcanos/metabolismo, Escherichia coli/genética, Escherichia coli/metabolismo, Fumarato Hidratasa/química, Fumarato Hidratasa/genética, Fumarato Hidratasa/metabolismo, Expresión Génica, NADP/química, NADP/metabolismo, Oxígeno/química, Oxígeno/metabolismo, Pliegue de Proteína, Proteínas Recombinantes/química, Proteínas Recombinantes/genética, Proteínas Recombinantes/metabolismo