In the first chapter, studies on substrate recognition and enzymatic activity of GGDEF domains are presented. Many proteins containing GGDEF domains are diguanylate cyclases (DGCs, EC 2.7.7.65), enzymes that catalyze the conversion of 2 GTP molecules into the second messenger c-di-GMP in prokaryotes. Thi...
Análisis de Secuencia,
Bacillus pumilus/clasificación,
Caulobacter crescentus/química,
Cianuros/efectos adversos,
Cinética,
Clonación de Organismos/instrumentación,
Concentración de Iones de Hidrógeno,
Especificidad por Sustrato,
Estilo de Vida,
Genoma Bacteriano,
Aislamiento de Pacientes,
Mutación Puntual
O objetivo deste comunicado é desenvolver um método quantitativo PCR em tempo real, baseado em guia molecular (MB) (MB-qPCR) para detecção de infecção por espécies de Brucella, e avaliar seu potencial de utilização clínica. Os primers e as sondas MB foram desenhados para amplificação específ...
Abstract INTRODUCTION: Tuberculosis is listed among the top 10 causes of deaths worldwide. The resistant strains causing this disease have been considered to be responsible for public health emergencies and health security threats. As stated by the World Health Organization (WHO), around 558,000 differe...
Klebsiella pneumoniae é um patógeno oportunista que causa principalmente infecções respiratórias e do trato urinário. A ocorrência frequente de isolados virulentos resistentes a múltiplas drogas levou a inclusão dessa espécie na lista da OMS das principais prioridades para pesquisa e desenvolvi...
ABSTRACT The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an "eco-friendly agricultural practice". Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea (Vigna unguiculata) inoculation in Brazil and showed remarkable r...
Inoculantes Agrícolas/clasificación,
Inoculantes Agrícolas/genética,
Inoculantes Agrícolas/aislamiento & purificación,
Inoculantes Agrícolas/fisiología,
Bradyrhizobium/clasificación,
Bradyrhizobium/genética,
Bradyrhizobium/aislamiento & purificación,
Bradyrhizobium/fisiología,
Brasil,
ADN Bacteriano/genética,
Evolución Molecular,
Genoma Bacteriano,
Genómica,
Filogenia,
ARN Ribosómico 16S/genética,
Nódulos de las Raíces de las Plantas/microbiología,
Simbiosis,
Vigna/microbiología,
Vigna/fisiología
Abstract Paraburkholderia tropica (syn Burkholderia tropica) are nitrogen-fixing bacteria commonly found in sugarcane. The Paraburkholderia tropica strain Ppe8 is part of the sugarcane inoculant consortium that has a beneficial effect on yield. Here, we report a draft genome sequence of this strain eluci...
Proteínas Bacterianas/genética,
Burkholderiaceae/genética,
Burkholderiaceae/aislamiento & purificación,
Biología Computacional,
Endófitos/genética,
Endófitos/aislamiento & purificación,
Genoma Bacteriano,
Redes y Vías Metabólicas/genética,
Anotación de Secuencia Molecular,
Saccharum/microbiología,
Análisis de Secuencia de ADN
Abstract Streptomycetes remain as one of the important sources for bioactive products. Isolated from the mangrove forest, Streptomyces gilvigriseus MUSC 26T was previously characterised as a novel streptomycete. The high quality draft genome of MUSC 26T contained 5,213,277 bp with G + C content of 73.0%....
Composición de Base,
Productos Biológicos/metabolismo,
Biología Computacional,
Microbiología Ambiental,
Genoma Bacteriano,
Redes y Vías Metabólicas/genética,
Metabolismo Secundario,
Análisis de Secuencia de ADN,
Streptomyces/genética,
Streptomyces/aislamiento & purificación,
Humedales
Background: Draft and complete genome sequences from bacteria are key tools to understand genetic determinants involved in pathogenesis in several disease models. Piscirickettsia salmonis is a Gram-negative bacterium responsible for the Salmon Rickettsial Syndrome (SRS), a bacterial disease that threaten...
ABSTRACT In this report, we present a draft genome of 2,886,173 bp of an Exiguobacterium aurantiacum strain PN47 isolate from the sediment of a saline pond named "Salar del Huasco" in the Altiplano in the North of Chile. Strain PN47 encodes adaptive characteristics enabling survival in extreme environmen...
Bacillaceae/clasificación,
Bacillaceae/genética,
Bacillaceae/aislamiento & purificación,
Bacillaceae/metabolismo,
Secuencia de Bases,
Chile,
ADN Bacteriano/genética,
Genoma Bacteriano,
Metales Pesados/análisis,
Metales Pesados/metabolismo,
Filogenia,
Estanques/química,
Estanques/microbiología,
Cloruro de Sodio/análisis,
Cloruro de Sodio/metabolismo
ABSTRACT As the largest genus in Actinobacteria family, Streptomyces species have the ability to synthesize numerous compounds of diverse structures with bioactivities. Streptomyces mangrovisoli MUSC 149T was previously isolated as a novel streptomycete from mangrove forest in east coast of Peninsular Ma...
Proteínas Bacterianas/genética,
Proteínas Bacterianas/metabolismo,
Composición de Base,
Secuencia de Bases,
ADN Bacteriano/genética,
Genoma Bacteriano,
Sedimentos Geológicos/microbiología,
Malasia,
Datos de Secuencia Molecular,
Filogenia,
Streptomyces/clasificación,
Streptomyces/genética,
Streptomyces/aislamiento & purificación
