Improving the expression of recombinant pullulanase by increasing mRNA stability in Escherichia coli
Electron. j. biotechnol; 29 (), 2017
Background: Pullulanase production in both wild-type strains and recombinantly engineered strains remains low. The Shine-Dalgarno (SD) sequence and stem-loop structure in the 5′ or 3′ untranslated region (UTR) are well-known determinants of mRNA stability. This study investigated the effect of mRNA s...