BACKGROUND: LXYL-P1-2 is the first reported glycoside hydrolase that can catalyze the transformation of 7-b-xylosyl-10-deacetyltaxol (XDT) to 10-deacetyltaxol (DT) by removing the D-xylosyl group at the C7 position. Successful synthesis of paclitaxel by one-pot method combining the LXYL-P1-2 and 10- deac...
Background: Burdock (Arctium lappa L.) is a fructan-rich plant with prebiotic potential. The aim of this study was to develop an efficient enzymatic route to prepare fructooligosaccharides (FOS)-rich and highly antioxidative syrup using burdock root as a raw material. Results: Endo-inulinase significantl...
Oligosacáridos/química,
Oligosacáridos/metabolismo,
Fructosa/química,
Fructosa/metabolismo,
Antioxidantes/química,
Antioxidantes/metabolismo,
Raíces de Plantas/química,
Arctium,
Alimentos Funcionales,
Cromatografía Líquida de Alta Presión,
Glicósido Hidrolasas/metabolismo,
Radical Hidroxilo,
Poligalacturonasa/metabolismo,
Hidrolasas de Éster Carboxílico/metabolismo,
Polifenoles
Background: α-L-Arabinofuranosidase (EC 3.2.1.55) catalyzes the hydrolysis of terminal α-L-1,2-, -1,3-, and -1,5- arabinofuranosyl residues in arabinose-containing polymers, and hence, it plays an important role in hemicellulose degradation. Herein, the bacterium Paenibacillus polymyxa, which secretes ...
Background: A new ι-carrageenase-producing strain was screened from mangroves and authenticated as Pseudoalteromonas carrageenovora ASY5 in our laboratory. The potential application of this new strain was evaluated. Results: Medium compositions and culturing conditions in shaking flask fermentation were...
Hidrólisis,
Pseudoalteromonas/enzimología,
Glicósido Hidrolasas/metabolismo,
Proteínas Bacterianas/metabolismo,
Fermentación,
Temperatura,
Concentración de Iones de Hidrógeno,
Carragenina/biosíntesis,
Espectrometría de Masa por Ionización de Electrospray,
Nitrógeno/metabolismo,
Carbono/metabolismo,
Oligosacáridos/biosíntesis
Background: Pullulanase production in both wild-type strains and recombinantly engineered strains remains low. The Shine-Dalgarno (SD) sequence and stem-loop structure in the 5′ or 3′ untranslated region (UTR) are well-known determinants of mRNA stability. This study investigated the effect of mRNA s...
Background: Current commercial production of isomalto-oligosaccharides (IMOs) commonly involves a lengthy multistage process with low yields. Results: To improve the process efficiency for production of IMOs, we developed a simple and efficient method by using enzyme cocktails composed of the recombinant...
Isomaltosa/metabolismo,
Oligosacáridos/metabolismo,
Enzimas/metabolismo,
Almidón/metabolismo,
alfa-Amilasas/metabolismo,
beta-Amilasa/metabolismo,
Glucosidasas/metabolismo,
Bacillus/enzimología,
Aspergillus niger/enzimología,
Oligosacáridos/biosíntesis,
Licuefacción,
Concentración de Iones de Hidrógeno,
Glicosilación,
Temperatura,
Glicósido Hidrolasas/metabolismo,
Fermentación
Background: A simple and efficient strategy for agarase immobilization was developed with carboxyl-functionalized magnetic nanoparticles (CMNPs) as support. The CMNPs and immobilized agarase (agarase-CMNPs) were characterized by transmission electron microscopy, dynamic light scattering, vibrating sample...
Glicósido Hidrolasas/metabolismo,
Enzimas Inmovilizadas,
Nanopartículas de Magnetita/química,
Dispersión Dinámica de Luz,
Termogravimetría,
Microscopía Electrónica de Transmisión,
Magnetometría,
Difracción de Rayos X,
Estabilidad de Enzimas,
Oligosacáridos/metabolismo,
Catálisis,
Glicósido Hidrolasas/química
Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as in...
A myrosinase (thioglucoside glucohydrolase or thioglucosidase, EC 3.2.3.147) producing fungus, Aspergillus sp. NR4617, was newly isolated from decayed soil sample obtained in Thailand and was subjected to single exposure to two chemical mutagens, ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitro...
Aspergillus/enzimología,
Aspergillus/genética,
Glicósido Hidrolasas/metabolismo,
Mutagénesis,
Planta de la Mostaza,
Metilnitronitrosoguanidina,
Metanosulfonato de Etilo/metabolismo,
Estabilidad de Enzimas,
Semillas,
Técnica del ADN Polimorfo Amplificado Aleatorio,
Suelo
Rhodotorula glutinis is a yeast that secretes the enzyme alpha-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the...
