Purification of inulinases by changing the ionic strength of the medium and precipitation with alcohols
An. acad. bras. ciênc; 89 (1), 2017
ABSTRACT The present study evaluated the purification of inulinase by changing the ionic strength of the medium by addition of NaCl and CaCl2 followed by precipitation with n-propyl alcohol or iso-propyl alcohol. The effects of the concentration of alcohols and the rate of addition of alcohols in the cru...
Alcoholes/química, Cloruro de Calcio/química, Precipitación Química, Medios de Cultivo/química, Glicósido Hidrolasas/química, Glicósido Hidrolasas/aislamiento & purificación, Kluyveromyces/química, Kluyveromyces/aislamiento & purificación, Concentración Osmolar, Valores de Referencia, Reproducibilidad de los Resultados, Sales (Química)/química, Cloruro de Sodio/química, Solventes/química