LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Glycoprotein nonmetastatic melanoma protein B accelerates tumorigenesis of cervical cancer in vitro by regulating the Wnt/β-catenin pathway

Xu, Shuxiang; Fan, Yingying; Li, Dongping; Liu, Yan; Chen, Xu.

Braz. j. med. biol. res; 52 (1), 2019

Cervical cancer is one of the most common cancers among women around the world. However, the underlying mechanism involved in cervical cancer progression is incompletely known. In the present study, we determined the role of glycoprotein nonmetastatic melanoma protein B (GPNMB) in tumorigenesis of cervic...

Apoptosis, Western Blotting, Línea Celular Tumoral, Movimiento Celular, Proliferación Celular, Regulación Neoplásica de la Expresión Génica/genética, Metaloproteinasa 2 de la Matriz/metabolismo, Metaloproteinasa 9 de la Matriz/metabolismo, Glicoproteínas de Membrana/genética, Glicoproteínas de Membrana/metabolismo, ARN Interferente Pequeño/metabolismo, Neoplasias del Cuello Uterino/genética, Neoplasias del Cuello Uterino/metabolismo, Neoplasias del Cuello Uterino/patología, Vía de Señalización Wnt/genética, beta Catenina/genética, beta Catenina/metabolismo

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Arsenic trioxide induces cell cycle arrest and affects Trk receptor expression in human neuroblastoma SK-N-SH cells

Xiong, Xilin; Li, Yang; Liu, Ling; Qi, Kai; Chen, Yueqin; Fang, Jianpei; Zhang, Chi.

Biol. Res; 51 (), 2018

BACKGROUND: Arsenic trioxide (As2O3), a drug that has been used in China for approximately two thousand years, induces cell death in a variety of cancer cell types, including neuroblastoma (NB). The tyrosine kinase receptor (Trk) family comprises three members, namely TrkA, TrkB and TrkC. Various studies...

Arsenicales/farmacología, Puntos de Control del Ciclo Celular/efectos de los fármacos, Proliferación Celular/efectos de los fármacos, Regulación Neoplásica de la Expresión Génica/efectos de los fármacos, Glicoproteínas de Membrana/efectos de los fármacos, Neuroblastoma/metabolismo, Óxidos/farmacología, Receptor trkB/efectos de los fármacos, Línea Celular Tumoral/efectos de los fármacos, Línea Celular Tumoral/metabolismo, Glicoproteínas de Membrana/metabolismo, Neuroblastoma/patología, Receptor trkB/metabolismo, Trióxido de Arsénico

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Suppression of miR-181 a attenuates H2O2-induced death of mesenchymal stem cells by maintaining hexokinase II expression

Lee, Seahyoung; Yun, Ina; Ham, Onju; Lee, Se-Yeon; Lee, Chang Yeon; Park, Jun-Hee; Lee, Jiyun; Seo, Hyang-Hee; Choi, Eunhyun; Hwang, Ki-Chul.

Biol. Res; 48 (), 2015

BACKGROUND: Low survival rate of transplanted cells compromises the efficacy of cell therapy. Hexokinase II (HKII) is known to have anti-apoptotic activity through its interaction with mitochondria. The objective was to identify miRNAs targeting HKII and investigate whether miRNA-mediated modulation of H...

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Development of a novel method to evaluate sialylation of glycoproteins and analysis of gp96 sialylation in Hela, SW1990 and A549 cell lines

Liang, Yangui; Hua, Qiang; Pan, Pengwei; Yang, Jie; Zhang, Qi.

Biol. Res; 48 (), 2015

BACKGROUND: Glycoproteins play a critical role in the cellular activities of eukaryotes. Sialic acid is typically the outermost monosaccharide of glycolipids and glycoproteins, and is necessary for normal development. RESULTS: A strategy based on avidin-biotin affinity was established to enrich sialylate...

Glicosiltransferasas/metabolismo, Glicoproteínas de Membrana/metabolismo, Proteínas de Neoplasias/metabolismo, Ácidos Siálicos/metabolismo, Células A549, Ensayo de Inmunoadsorción Enzimática, Células HeLa, Reacción en Cadena en Tiempo Real de la Polimerasa, Espectrometría de Masas en Tándem

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Functional disruption of human leukocyte antigen II in human embryonic stem cell

Chen, Haide; Li, Yang; Lin, Xijuan; Cui, Di; Cui, Chun; Li, Hui; Xiao, Lei.

Biol. Res; 48 (), 2015

BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact sam...

Diferenciación Celular/genética, Desoxirribonucleasas/metabolismo, Células Madre Embrionarias Humanas/metabolismo, Proteínas Nucleares/genética, Transactivadores/genética, Eliminación de Gen, Células Presentadoras de Antígenos/metabolismo, Antígenos CD/metabolismo, Antígeno B7-2/metabolismo, Células Dendríticas/metabolismo, Desoxirribonucleasas/clasificación, Cuerpos Embrioides/metabolismo, Fibroblastos/metabolismo, Antígenos de Histocompatibilidad Clase II/genética, Inmunoglobulinas/metabolismo, Interferón gamma/metabolismo, Glicoproteínas de Membrana/metabolismo, Autorrenovación de las Células, Inmunohistoquímica, Cariotipo, Ratones SCID, Reacción en Cadena en Tiempo Real de la Polimerasa, Reacción en Cadena de la Polimerasa de Transcriptasa Inversa, Teratoma, Células Tumorales Cultivadas

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