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Cyclodextrin glucanotransferase immobilization onto functionalized magnetic double mesoporous core-shell silica nanospheres

Ibrahim, Abdelnasser S. S; Al-Salamah, Ali A; El-Toni, Ahmed Mohamed; El-Tayeb, Mohamed A; Elbadawi, Yahya B.
Electron. j. biotechnol; 17 (2), 2014
Background Cyclodextrin glucanotransferase (CGTase) from Amphibacillus sp. NPST-10 was covalently immobilized onto amino-functionalized magnetic double mesoporous core-shell silica nanospheres (mag@d-SiO2@m-SiO2-NH2), and the properties of the immobilized enzyme were investigated. The synthesis process o...
Glucosiltransferasas/metabolismo, Enzimas Inmovilizadas, Bacillaceae/enzimología, Nanosferas, Dióxido de Silicio, Porosidad, Ciclodextrinas, Solventes/aislamiento & purificación, Glucosiltransferasas/aislamiento & purificación, Glucosiltransferasas/biosíntesis, Temperatura, Concentración de Iones de Hidrógeno
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A new low molecular mass alkaline cyclodextrin glucanotransferase from Amphibacillus sp. NRC-WN isolated from an Egyptian soda lake

Al-Sharawi, Samar Z. R; Ibrahim, Abdelnasser S. S; El-Shatoury, Einas H; Gebreel, Hassan M; Eldiwany, Ahmad.
Electron. j. biotechnol; 16 (6), 2013
Background: Cyclodextrin glucanotransferase (CGTase) is one of the most industrially important enzymes used in the commercial production of cyclodextrins (CDs). Alkaliphilic bacteria have attracted much interest in the last few decades because of their ability to produce extracellular enzymes that are ac...
Bacillaceae/enzimología, Glucosiltransferasas/biosíntesis, Lagos/microbiología, Bacillaceae/aislamiento & purificación, Adsorción, Cromatografía por Intercambio Iónico, Temperatura, Concentración de Iones de Hidrógeno, Cinética, Peso Molecular, Estabilidad de Enzimas, Glucosiltransferasas/metabolismo
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Immobilization of cyclodextrin glucanotransferase on aminopropyl-functionalized silica-coated superparamagnetic nanoparticles

Ibrahim, Abdelnasser S. S; Al-Salamah, Ali A; El-Toni, Ahmed Mohamed; El-Tayeb, Mohamed A; Elbadawi, Yahya B.
Electron. j. biotechnol; 16 (6), 2013
Background: Cyclodextrin glycosyltransferase (CGTase) from Amphibacillus sp. NPST-10 was successfully covalently immobilized on aminopropyl-functionalized silica coated superparamagnetic nanoparticles; and the properties of immobilized enzyme were investigated. The synthesis process included preparing of...
Glucosiltransferasas/química, Enzimas Inmovilizadas/química, Nanopartículas de Magnetita/química, Dióxido de Silicio, Bacillaceae/enzimología, Técnicas de Cultivo, Glucosiltransferasas/biosíntesis, Glucosiltransferasas/aislamiento & purificación, Concentración de Iones de Hidrógeno, Temperatura, Cinética, Glucosiltransferasas/metabolismo, Enzimas Inmovilizadas/metabolismo, Ciclodextrinas, Espectrofotometría Infrarroja
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