Molecular cloning, expression, and immobilization of glutamate decarboxylase from Lactobacillus fermentum YS2
Electron. j. biotechnol; 27 (), 2017
Background: GABA (γ-aminobutyric acid) is a four-carbon nonprotein amino acid that has hypotensive, diuretic, and tranquilizing properties. Glutamate decarboxylase (GAD) is the key enzyme to generate GABA. A simple and economical method of preparing and immobilizing GAD would be helpful for GABA product...
Limosilactobacillus fermentum/enzimología, Glutamato Descarboxilasa/metabolismo, Glutamato Descarboxilasa/genética, Clonación Molecular, Enzimas Inmovilizadas, Escherichia coli, Ácido gamma-Aminobutírico/biosíntesis, Proteínas Recombinantes de Fusión, Celulosa, Adsorción, Temperatura, Concentración de Iones de Hidrógeno