Results: 8

Características operativas de técnicas moleculares empleadas para la detección del virus del papiloma humano en el proyecto ESTAMPA

Rev. colomb. cancerol; 24 (3), 2020
Resumen El desarrollo y la innovación de nuevas tecnologías ha permitido mejorar la detección de la infección por el virus del papiloma humano de alto riesgo. La captura de híbridos II es un ensayo que se basa en hibridación y quimioluminiscencia. Cobas VPH Test es una PCR cualitativa y Aptima VPH ...

Successful and failed mini-implants: microbiological evaluation and quantification of bacterial endotoxin

J. appl. oral sci; 26 (), 2018
Abstract Objectives Using two groups of mini-implants (successful and failed) the objectives of this in vivo study were: to evaluate the microbial contamination by the checkerboard DNA-DNA hybridization technique and to quantify the bacterial endotoxin by the limulus amebocyte lysate assay. Material a...

Utilization of the oncoscan microarray assay in cancer diagnostics

Appl. cancer res; 37 (), 2017
Current strategies for cancer patient management include the use of genomic and proteomic test results to help guide therapeutic selection. The need for multi-target variant analysis is highlighted by the growing number of novel therapies to treat tumors with specific profiles and the increasing recognit...

Diagnóstico molecular do papilomavírus humano por captura híbrida e reação em cadeia da polimerase

Femina; 43 (4), 2015
A infecção pelo papilomavírus humano (HPV) é extremamente comum e está associada a várias condições clínicas, que variam de infecções assintomáticas a doenças benignas e malignas da mucosa genital, como as verrugas genitais, a neoplasia intraepitelial cervical e o câncer do colo do útero. ...

Isolation and characterization of drought-responsive genes from peanut roots by suppression subtractive hybridization

Electron. j. biotechnol; 17 (6), 2014
Background Peanut (Arachis hypogaea L.) is an important economic and oilseed crop. Long-term rainless conditions and seasonal droughts can limit peanut yields and were conducive to preharvest aflatoxin contamination. To elucidate the molecular mechanisms by which peanut responds and adapts to water limit...

Analysis of gene expression profiles in response to Sporisorium reilianum f. sp. zeae in maize (Zea mays L.)

Electron. j. biotechnol; 17 (5), 2014
Background Head smut of maize, which is caused by Sporisorium reilianum f. sp. zeae (Kühn), is a serious disease in maize. In order to reveal the molecular mechanism of the resistance to head smut in maize, a microarray containing ~ 14,850 probes was used to monitor the gene expression profiles between ...

Analysis of gene expression in Kalanchoe daigremontiana leaves during plantlet formation under drought stress

Electron. j. biotechnol; 16 (6), 2013
Background: Kalanchoe daigremontiana is an attractive model system for the study of the molecular mechanisms of somatic embryogenesis and organogenesis competence due to its formation of plantlets with adventitious roots on the leaf margins that are derived from somatic embryos. The suppression subtracti...

Patología molecular y diagnóstico de enformedades infecciosas

Rev. méd. hondur; 59 (3), 1991
Los autores revisan y describe métodos moleculares para la detección de agentes etiológicos o secuencias genéticas involucradas en la patogénesis de varias enfermedades. Hay sondas moleculares ya disponibles para el diagnóstico rápido de enfermedades causadas por un gran número de virus, bacteria...