Home > LILACS - Literatura Latinoamericana y del Caribe de Ciencias de la Salud > Search result

Results: 3

Optimization of expression yield in a stable cell line expressing a novel mutated chimeric tissue plasminogen activator (mt-PA)

Raigani, Mozhgan; Barkhordari, Farzaneh; Moazzami, Reza; Davami, Fatemeh; Mahboudi, Fereidoun.
Braz. J. Pharm. Sci. (Online); 58 (), 2022
Abstract The development of stable cell lines producing recombinant proteins is very time-consuming and laborious. One of the practical approaches successfully performed is Fluorescence-Activated Cell Sorting (FACS). A mutated chimeric tissue plasminogen activator (mt-PA) was developed by removing the fi...
Activador de Tejido Plasminógeno, Optimización de Procesos, Fluorescencia, Citometría de Flujo/métodos, Recuento de Células/instrumentación, Células Clonales/clasificación, Inhibidor 1 de Activador Plasminogénico/efectos adversos, Proteínas Fluorescentes Verdes
Fulltext

Decreased expression of microRNA-17 in hippocampal tissues and blood from mice with depression up-regulates the expression of PAI-1 mRNA and protein

Lv, Min; Li, Jing; Gao, Xinxue; Hao, Yurong; Zhao, Fengxia.
Braz. j. med. biol. res; 53 (10), 2020
This study determined the expression of plasminogen activator inhibitor-1 (PAI-1) and microRNA (miR)-17 in a mouse depression model. Forty male mice were divided evenly into control and depression groups. A chronic unpredictable mild stress (CUMS) model was constructed. qRT-PCR was used to determine the ...
Depresión/metabolismo, Hipocampo/metabolismo, MicroARNs, Inhibidor 1 de Activador Plasminogénico, ARN Mensajero
Fulltext

PAI-1 haplogenotype confers genetic susceptibility for obesity and hypertriglyceridemia in Mexican children.

de la Cruz-Mosso, Ulises; Ramos-Arellano, Luz Elena; Muñoz-Valle, José Francisco; Salgado-Bernabé, Aralia Berenice; Salgado-Goytia, Lorenzo; Castro-Alarcón, Natividad; Ramírez-Peralta, Arturo; Parra-Rojas, Isela.
Invest. clín; 57 (3), 2016
The presence of childhood obesity predisposes to the development of cardio vascular and metabolic diseases, such as coronary artery disease and type 2 diabetes mellitus, in adulthood. The polymorphisms described in PAI-1 gene have been linked with obesity and metabolic syndrome in several populations. Th...
Hipertrigliceridemia/genética, Obesidad Infantil/genética, Inhibidor 1 de Activador Plasminogénico/genética, Estudios Transversales, Predisposición Genética a la Enfermedad, Genotipo, México
Fulltext