Comparison of senescence progression in mesenchymal cells from human umbilical cord walls measured by immunofluorescence and flow cytometry of p16 and p21
Einstein (São Paulo, Online); 18 (), 2020
ABSTRACT Objective To follow the expansion of mesenchymal stem cells from umbilical cords by two classic senescence markers, p16 (INK4A) and p21 (CDKN1A), using practical, fast, and less expensive methods than the gold standard Western blotting technique, to evaluate its applicability in the laboratory...
Biomarcadores/sangre, Western Blotting, Células Cultivadas, Senescencia Celular, Inhibidor p16 de la Quinasa Dependiente de Ciclina, Inhibidor p21 de las Quinasas Dependientes de la Ciclina, Citometría de Flujo/métodos, Técnica del Anticuerpo Fluorescente/métodos, Células Madre Mesenquimatosas/fisiología, Cordón Umbilical/fisiología