LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Overexpression of a cysteine proteinase inhibitor gene from Jatropha curcas confers enhanced tolerance to salinity stress

Background Cysteine proteinase inhibitor (cystatin, CPI) is one of the most important molecules involved in plant development and defense, especially in the regulation of stress responses. However, it is still unclear whether the Jatropha curcas CPI (JcCPI) gene functions in salinity response and toleran...

Simultaneous silencing of VEGF and KSP by siRNA cocktail inhibits proliferation and induces apoptosis of hepatocellular carcinoma Hep3B cells

Doan, Chung Chinh; Le, Long Thanh; Hoang, Son Nghia; Do, Si Minh; Van Le, Dong.

Biol. Res; 47 (), 2014

BACKGROUND: Vascular endothelial growth factor (VEGF) is involved in the growth of new blood vessels that feed tumors and kinesin spindle protein (KSP) plays a critical role in mitosis involving in cell proliferation. Simultaneous silencing of VEGF and KSP, an attractive and viable approach in cancer, le...

Apoptosis/genética, Proliferación Celular/genética, Silenciador del Gen, Cinesinas/genética, ARN Interferente Pequeño/genética, Factor A de Crecimiento Endotelial Vascular/genética, Ciclina D1/metabolismo, Inhibidores de Cisteína Proteinasa/metabolismo, Células Endoteliales de la Vena Umbilical Humana/metabolismo, Proteínas Inhibidoras de la Apoptosis/metabolismo, Cinesinas/metabolismo, Mitosis/genética, Factor A de Crecimiento Endotelial Vascular/metabolismo, Proteínas de Transporte Vesicular/metabolismo, Anexina A5, Western Blotting, Línea Celular Tumoral, Movimiento Celular, Regulación hacia Abajo, Citometría de Flujo, Genes bcl-2, Reacción en Cadena en Tiempo Real de la Polimerasa, Survivin, Sales de Tetrazolio, Transfección

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