LILACS – Literatura Latinoamericana y del Caribe de Ciencias de la Salud

Production and evaluation of egg derived hot start antibodies

Spyrydonov, Vladyslav; Pihida, Dmitro; Sereda, Alexander; Likhanov, Artur; Yu, Weiming.

Electron. j. biotechnol; 44 (), 2020

BACKGROUND: Hot start can greatly improve specificity, sensitivity and yield of PCR. Non-specific amplification can occur in PCR when reaction mixture is prepared at room temperature, because Taq DNA polymerase is active and the primers can hybridize non-specifically. Hot start Taq DNA polymerases remain...

Anticuerpos Monoclonales/inmunología, Yema de Huevo/inmunología, Temperatura, Ensayo de Inmunoadsorción Enzimática, Polimerasa Taq, Inmunoglobulinas/inmunología, Reacción en Cadena de la Polimerasa, Anticuerpos Monoclonales/aislamiento & purificación, Inmunoglobulinas/aislamiento & purificación, Yema de Huevo/metabolismo, Anticuerpos Monoclonales/biosíntesis, Inmunoglobulinas/biosíntesis

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Comparison of techniques of detecting immunoglobulin-binding protein reactivity to immunoglobulin produced by different avian and mammalian species

The rationale of this study was to use several immunological assays to investigate the reactivity of immunoglobulin binding protein (IBP) to immunoglobulins from various avian and mammalian species. The IBP studied were Staphylococcal protein A (SpA), Streptococcal protein G (SpG), Peptostreptococcal pro...